Plevral epiteloid malign mezotelyoma ile adenokarsinom ayirici tanisinda immunhistokimya; bir panel onerisi / Immunohistochemistry for differentiation of pleural epithelioid malignant mesothelioma from adenocarcinoma: a panel for selection.

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Authors: Elif Ulker Akyildiz, Buge Oz, Hilal Aki and Ahmet Demirkaya
Date: Dec. 2010
From: Turkish Thoracic Journal(Vol. 11, Issue 4)
Publisher: AVES
Document Type: Article
Length: 2,219 words

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Amac: Toraks patolojisi pratiginde karsimiza cikan onemli sorunlardan biri plevral epiteloid malign mezotelyoma ile plevrayi infiltre eden pulmoner adenokarsinomun ya da metastatik adenokarsinomun ayirici tanisinin yapilmasidir. Epiteloid malign mezotelyoma, adenokarsinoma benzer mikroskopik paternler gosterebildiginden ayirici tanida morfolojik bulgular yetersiz kalmaktadir. Bu ayirimin yapilabilmesi icin gunumuze degin pek cok immunhistokimyasal isaretleyici one surulmus ve her biri icin sensitivite ve spesifiteyi arastiran cok sayida makale yayinlanmistir.

Gerec ve Yontem: Bu calismada bolumumuzde son 7 yil icinde tani almis plevral epitelyal malign mezotelyoma olgulari ile plevrayi infiltre etmis adenokarsinom olgularina ait raporlar incelenmis bu olgularda ayirici taniya giderken kullanilan immunhistokimyasal isaretleyiciler degerlendirilmistir.

Bulgular: En sik kullanilan isaretleyiciler epiteloid malign mezotelyoma icin kalretinin ve CK5/6, adenokarsinom icin CEA, CD15 ve TTF-1 idi. Her iki grupda da EMA calisilmisti.

Sonuc: Laboratuarimizda epiteloid malign mezotelyoma acisindan kalretinin, CK5/6, adenokarsinom acisindan CEA ve CD15 ve bunlara eklenen EMA ve TTF-1'den olusan bir panelin hemen tum olgularda sorunu cozdugunu gorduk. Uygulanan bu panelin sonuclannda bir uyumsuzluk izlendigi takdirde diger antikorlarin eklenmesinin uygun olacagini dusunmekteyiz.

(Tur Toraks Der 2010; 11: 144-8)

Anahtar sozcukler: Malign mezotelyoma, adenokarsinoma, immunhistokimya

Gelis Tarihi: 08.04.2009

Kabul Tarihi: 22.12.2009

ABSTRACT

Objective: An important difficulty pathologists confront concerning thoracic pathology is the differentiation of pleural epithelioid malignant mesothelioma from pulmonary adenocarcinoma infiltrating into the pleura or metastatic adenocarcinoma. Since epithelioid malignant mesothelioma may depict microscopic patterns similar to adenocarcinoma, morphological findings may not be sufficient to make a differential diagnosis. Up to now, many immunohistochemical markers have been proposed for their differentiation and there have been many studies on the sensitivity and specifity of each marker.

Material and Method: In this study we retrospectively evaluated the reports on patients diagnosed as pleural epithelial MM and patients diagnosed as adenocarcinoma infiltrating into the pleura for the past seven years and determined the immunohistochemical markers used for differential diagnosis.

Results: Most frequently used markers were calretinin and CK5/6 for epithelioid malignant mesothelioma and CEA, CD1 5 and TTF-1 for adenocarcinoma. EMA was used in both groups.

Conclusion: We found that a panel including calretinin and CK5/6 for malignant mesothelioma and CEA and CD15 for adenocarcinoma and additionally EMA and TTF-1 avoided the difficulty in all cases. We consider that, if there is any inconsistency between the results of staining with these six markers, other antibodies should be incorporated into the panel.

(Tur Toraks Der 2010; 11: 144-8)

Key words: Malignant mesothelioma, adenocarcinoma, immunohistochemistry

Received: 08.04.2009

Accepted: 22.12.2009

GIRIS

Toraks patolojisi pratiginde karsimiza cikan onemli sorunlardan biri, plevral epiteloid malign mezotelyoma (MM) ile plevrayi infiltre eden pulmoner adenokarsinom ya da metastatik adenokarsinomun ayirici tanisinin yapilmasidir Epiteloid MM, adenokarsinom benzeri mikroskopik paternler gosterebildiginden ayirici tanida morfolojik bulgular yetersiz kalmakta, immunhistokimyasal yontemlerin uygulanmasi gerekmektedir. Bu ayirimin yapilabilmesi icin gunumuze degin pek cok immunhistokimyasal isaretleyici one surulmus ve her bid icin sensitivite ve spesifiteyi arastiran cok sayida makale yayinlanmistir (1-6). Bu isaretleyicilerden bazilari; kalretinin, CK5/6, mesotelin, Wilms' tumor product-1 (WT-1), trombomodulin gibi mezotel hucrelerine yonelik isaretleyiciler iken bazilari karsinoembriyonik antijen (CEA), CD15, B72.3, MOC-31, Ber-Ep4 gibi adenokarsinom hucrelerini boyamaya yonelik isaretleyicilerdir....

Source Citation

Source Citation
Akyildiz, Elif Ulker, et al. "Plevral epiteloid malign mezotelyoma ile adenokarsinom ayirici tanisinda immunhistokimya; bir panel onerisi / Immunohistochemistry for differentiation of pleural epithelioid malignant mesothelioma from adenocarcinoma: a panel for selection." Turkish Thoracic Journal, vol. 11, no. 4, Dec. 2010, pp. 144+. Accessed 28 May 2022.
  

Gale Document Number: GALE|A305563060