The association between gene polymorphisms and leukocytosis with thrombotic complications in patients with essential thrombocythemia and polycythemia vera / Esansiyel Trombositemi ve Polisitemia Verada Gen Polimofizimleri ve Lokositozun Trombotik Komplikasyonlar ile Iliskisi

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Authors: Ozgur Mehtap, Elif Birtas Atesoglu, Pinar Tarkun, Emel Gonullu, Hakan Keski and Yildiray Topcu
Date: June 2012
From: Turkish Journal of Hematology(Vol. 29, Issue 2)
Publisher: Galenos Yayinevi Tic. Ltd.
Document Type: Report
Length: 4,871 words

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Abstract

Objective: Vascular events are a common complication in patients with polycythemia vera (PV) and essential thrombocythemia (ET). This study aimed to analyze the association between PAI-1 4G/5G and ACE I/D gene polymorphisms, and leukocytosis with thrombosis in patients with PV and ET.

Material and Methods: In total, 64 patients with ET and PV were evaluated. Arterial or venous thrombosis, such as cerebral transient ischemic attack, ischemic stroke, myocardial infarction, peripheral arterial thrombosis, deep venous thrombosis, and pulmonary embolism, were defined as a vascular event. DNA samples were screened for mutations via reverse hybridization strip assay.

Results: In terms of PAI-1 gene polymorphism, the frequency of the 4G and 5G allele was 48.5% and 51.5%, respectively. The ACE allele frequency was 51.2% and 48.8% for D and I, respectively. There wasn't an association between occurrence of vascular events and the frequency of any allele. In terms of occurrence of vascular events, there weren't any significance differences between the patients that were carrying the ACE D/D homozygous allele to ACE I/D and those that carried the I/I allele (P = 0.93). There wasn't a significant difference in occurrence of vascular events between the PAI-1 5G/5G homozygote allele carriers, and the 4G/5G and 4G/4G allele carriers (P = 0.97). Vascular events were significantly more common in the patients with leukocytosis (leukocyte count >10 x [10.sup.9] [L.sup.-1]) than in those without leukocytosis (leukocyte count [10.sup.9] [L.sup.-1]) (P = 0.00). Age >60 years was also a significant risk factor for occurrence of vascular events(P = 0.008).

Conclusion: PAI-1 and ACE gene polymorphisms were not considered new risk factors for thrombosis in PV and ET patients. On the other hand, leukocytosis at diagnosis was associated with the occurrence of vascular events in the patients with ET and PV.

Key Words: PAI-1 4G/5G, ACE I/D, Polycythemia vera, Essential thrombocythemia, Thrombosis, Leukocytosis

Ozet

Amac: Polisitemia vera (PV) ve esansiyel trombositemi (ET)'de vaskuler olaylar sik gorulen komplikasyonlarthr. Bu ealismada, PV, ET hastalarinda PAI-1 4G/5G, ACE I/D gen polimorfizmi ile lokositoz ve bunlarin vaskuler olaylarla iliskisini arastirmari amacladik.

Gerec ve Yontemler: PV ve ET'li 64 hasta calismaya alindi. Serebral geciei iskemik atak, iskemik inme, miyokard infarktusu, periferik arteryel tromboz, derin ven trombozu ve pulmoner emboli gibi arteryel veya venoz trombozlar vaskuler olay olarak tanimlanmistir. DNA ornekleri mutasyonlar yonunden ters hibridizasyon strip testi ile arastirildi.

Bulgular: PAI-1 gen polimorfizmine bakildiginda 4G ve 5G allel sikligi sirasi ile %48,5 ve %52,5 tespit edildi. ACE geninin D ve I sikliklari strasi ile %51,2 ve %48,8 bulundu. Vaskuler olaylar ile allel sikliklari arasinda anlamli iliski saptamadik. Vaskuler olaylar degerlendirildiginde, ACE D/D homozigot allel tasiyan hastalar ile ACE I/D ve I/I allel tasiyanlar arasinda anlamli fark tespit edemedik (P = 0,93). PAI-1 5G/5G homozigot allel tasiyicilari ile 4G/5G ve 4G/4G allel tasiyicilari karsilastirildiginda vaskuler olay gorulmesi acisindan anlamli farklilik tespit edilmedi (P = 0,97).

Vaskuler olayin, lokositozu (>10x[10.sup.9]/L) olan hastalarda, lokositozu olmayanlara gore belirgin olarak daha sik goruldugu saptandi = 0,00). Ayriea tani anindaki lokositoz varliginin vaskuler olay riskini belirgin olarak arttirdigi tespit edildi...

Source Citation

Source Citation
Mehtap, Ozgur, et al. "The association between gene polymorphisms and leukocytosis with thrombotic complications in patients with essential thrombocythemia and polycythemia vera / Esansiyel Trombositemi ve Polisitemia Verada Gen Polimofizimleri ve Lokositozun Trombotik Komplikasyonlar ile Iliskisi." Turkish Journal of Hematology, vol. 29, no. 2, 2012, p. 162+. Accessed 8 Mar. 2021.
  

Gale Document Number: GALE|A345620991