Investigation of the Enteric Adenovirus Antigen Frequency by Immunochromotographic Method in Children with Acute Gastroenteritis/Akut Gastroenteric Cocuklarda Enterik Adenovirus Antijen Sikliginin Immunokromatografik Yontemle Arastirilmasi

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Authors: Orhan Akpinar and Hatice Akpinar
Date: Aug. 2017
From: Meandros Medical and Dental Journal(Vol. 18, Issue 2)
Publisher: Galenos Yayinevi Tic. Ltd.
Document Type: Report
Length: 2,231 words

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Abstract

Objective: Gastroenteritis is the third common cause of death due to infections. After rotavirus, adenoviruses are also one of the reasons frequently seen in gastroenteritis in infants and children. This study is performed to determine the incidence of enteric virus serotype 40 and 41 in children with acute gastroenteritis in order to enable prompt and appropriate treatment.

Materials and Methods: Stool specimens of patients who attended our clinic with a diagnosis of acute gastroenteritis between January 2013 and December 2013 were examined for the presence of enteric adenovirus (Ad40 and Ad41) antigen using immunochromatographic methods.

Results: One hundred and two stool samples from 3206 were positive for adenovirus antigens. Adenovirus antigen positive-patients aged 0-5 years constituted 82.3% of patients. Adenovirus infections were observed in all seasons of the year.

Conclusion: In our country, the epidemiology of adenovirus infection is not known very well. According to the data we obtained from the results of this study, we assume that idntifying viral agent in patients with diarrhea in an accurate, prompt and reliable way can prevent unnecessary antibiotic use and can contribute seroepidemiologic data in childhood gastroenteritis in our region.

Keywords Chilhood diarrhea, adenovirus, acute gastroenteritis, immunochromatographic

Öz

Amaç: Gastroenteritler enfeksiyonlara bagli ölümler arasinda ilk üç sirada yer almaktadir. Bebekler ve çocuklardaki gastroenteritlerin rotavirüslerden sonra diger sik görülen nedenlerinden biri de adenovirüslerdir. Bu çalisma, akut gastroenterit nedeniyle basvuran çocuk hastalarda enterik adenovirüs serotip 40 ve 41 insidansini inceleyerek uygun tedaviye kisa sürede baslanabilmesi amaciyla yapilmistir.

Gereç ve Yöntemler: Ocak 2013-Aralik 2013 tarihleri arasinda, akut gastroenterit tanisiyla basvuran 0-14 yas grubu hastalarin diski örneginde enterik adenovirüs (Ad40 ve Ad41) antijen varligi immünokromatografik yöntem kullanilarak arastirildi.

Bulgular: Üç bin ikiyüzalti diski örneginden 102'sinde (%3,2) adenovirus antijenleri saptandi. Sifir-bes yas arasi grup olgularin %82,3'ünü olusturmaktaydi. Adenovirüs enfeksiyonu yilin her mevsiminde görülmüstür.

Sonuç: Ülkemizde adenovirüs enfeksiyonu seroepidemiyolojisi çok iyi bilinmemektedir. Yaptigimiz bu çalisma sonucu elde ettigimiz verileri degerlendirdigimizde ishal sikayeti ile basvuran hastalarda viral etkenin dogru, hizli ve güvenilir bir sekilde tanimlanarak bölgemizde görülen çocukluk çagi gastroenteritlerinde seroepidemiyolojik verilere katkida bulunacagi ve gereksiz antibiyotik kullaniminin önüne geçilebilecegini düsünmekteyiz.

Anahtar Kelimeler Çocukluk çagi ishalleri, adenovirus, akut gastroenterit, immünokromotografik

Introduction

Diarrhea has emerged as a major health problem all over the world, especially in developing countries. Gastroenteritis is among the first three causes of deaths due to infections (1). Viruses are responsible for the vast majority of childhood diarrhea. Enteropathogenic viruses are the main factors blamed. Rotaviruses followed by adenoviruses are the major pathogens causing gastroenteritis in infants and children. Adenoviruses are double-stranded, non-enveloped DNA viruses. There are 51 different serotypes of adenoviruses and they can be classified into 6 subgenera (A-F). Certain serotypes show specific tissue tropism and lead to specific diseases. They can be found in the stool during and after upper respiratory tract infection but only subgenus F; serotype 40 and 41, more rarely serotype 31, cause gastroenteritis (2,3). Enteric adenoviruses (Ad40 and Ad41) that are acute gastroenteritis factors cause milder and self-limiting infection. However, they may become persistent and life-threatening in immunocompromised patients....

Source Citation

Source Citation
Akpinar, Orhan, and Hatice Akpinar. "Investigation of the Enteric Adenovirus Antigen Frequency by Immunochromotographic Method in Children with Acute Gastroenteritis/Akut Gastroenteric Cocuklarda Enterik Adenovirus Antijen Sikliginin Immunokromatografik Yontemle Arastirilmasi." Meandros Medical and Dental Journal, vol. 18, no. 2, 2017, p. 86+. Accessed 10 Apr. 2021.
  

Gale Document Number: GALE|A508842123