Neutralizing Potential of Fab IgG Hybrid Antibody Against Dengue Virus (DENV-1,2,3,4) Expressed on Mesenchymal Stem Cells/Mezenkimal Kok Hucreler Uzerinde Dengue Virusune Karsi (DENV-1,2,3,4) Fab IgG Hibrit Antikorunun Notralize Edici Potansiyeli

Citation metadata

Authors: Fedik A. Rantam, Rahaju Ernawati, Jola Rahmahani, Eryk Hendrianto, Helen Susilowati and Rofqul A'La
Date: Jan. 2018
From: Journal of the Faculty of Veterinary Medicine(Vol. 44, Issue 1)
Publisher: AVES
Document Type: Report
Length: 4,297 words

Main content

Abstract :

Dengue is an acute mosquito born viral infections in tropical and subtropical countries, that has been steadily increasing every year. Dengue haemorrhagic fever in Indonesia is caused by all 4 viral serotypes termed as DENV-1, DENV-2, DENV-3, and DENV-4. It is very dificult to treat the disease and there is no effective vaccine to eradicate Dengue virus infections. The aim of this research is to express the antibody fragment gene coding VL and VH of Fab IgG antibody in rat bone marrow mesenchymal stem cells (rat BM-MSCs). The genes were isolated from immunized mice using inactive virus of all dengue serotypes. Then genes were hybridized through ligation and insertion into plasmid pBR322, and transfected into rat BMMSCs. To analytically characterize Fab IgG hybrid binding ability, capacity and specificity, we used immune precipitation, western blotting, neutralization assay, and ELISA. All assays suggested that hybrid Fab IgG antibody have high reactivity and afinity, to eficiently neutralize all Dengue virus serotypes 1,2,3,4. Results of this study show that hybrid Fab IgG antibody can be used as neutralizing agent for the treatment of dengue infections in the future. Keywords: Dengue virus, FabIgG hybrid antibody, generated expression ratBMMSCs, reactivity, neutralization Dengue hummasi, tropik ve subtropikal ulkelerde sivrisineklerden kaynaklanan akut viral bir enfeksiyon olup, her yil giderek artmaktadir. Endonezya'daki Dang kanamali atesine DENV-1, DENV-2, DENV-3 ve DENV-4 adi verilen 4 viral serotip neden olmaktadir. Hastaligin tedavisi cok zordur ve Dang virusu enfeksiyonlarini yok etmek icin etkili bir asi yoktur. Bu arastirmanin amaci, sican kemik iligi mezenkimal kok hucrelerinde (sican BM-MSCs) bulunan Fab IgG antikorunun VL ve VH antikor fragmentini kodlayan geninin aciga cikarilmasidir. Genler, tum dang serotiplerinin inaktif virusu kullanilarak asilanmis farelerden izole edilmistir. Sonrasinda genler, ligasyon ve plasmid pBR322 icine gomulmesi yoluyla hibridize edilmis ve sican BMMSC'lerine nakledilmistir. Analitik olarak Fab IgG'nin hibrid baglama yetenegi, kapasitesi ve ozgunlugunu karakterize etmek icin; bagisiklik cokeltmesi, western blotting, notralizasyon testi ve ELISA kullanilmistir. Butun deneyler, hibrid Fab IgG antikorunun tum Dang virusu serotipleri 1,2,3,4'u etkili bir sekilde notrlestirmek icin yuksek reaktiviteye ve afiniteye sahip oldugunu ortaya koymaktadir. Bu calismanin sonuclari, melez Fab IgG antikorunun gelecekte dang enfeksiyonlarinin tedavisinde notralize edici ajan olarak kullanilabilecegini gostermektedir. Anahtar kelimeler: Dengue virusu, Fab IgG hibrid antikor, sican kemik iligi mezenkimal kok hucrelerinin ekspresyonu (ratBMMSC), reaktivite, notrlestirme

Source Citation

Source Citation
Rantam, Fedik A., et al. "Neutralizing Potential of Fab IgG Hybrid Antibody Against Dengue Virus (DENV-1,2,3,4) Expressed on Mesenchymal Stem Cells/Mezenkimal Kok Hucreler Uzerinde Dengue Virusune Karsi (DENV-1,2,3,4) Fab IgG Hibrit Antikorunun Notralize Edici Potansiyeli." Journal of the Faculty of Veterinary Medicine, vol. 44, no. 1, 2018, p. 12+. Accessed 13 May 2021.
  

Gale Document Number: GALE|A560416815