BTEX biodegradation and its nitrogen removal potential by a newly isolated Pseudomonas thivervalensis MAH1

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Authors: Dan Qu, Yongsheng Zhao, Jiaqiang Sun, Hejun Ren and Rui Zhou
Date: Sept. 1, 2015
From: Canadian Journal of Microbiology(Vol. 61, Issue 9.)
Publisher: NRC Research Press
Document Type: Report
Length: 5,279 words
Abstract :

Benzene, toluene, ethylbenzene, and xylene (BTEX) are of great environmental concern because of their widespread occurrence in groundwater and soil, posing an increasing threat to human health. The aerobic denitrifying BTEX-degrading bacterium Pseudomonas thivervalensis MAH1 was isolated from BTEX-contaminated sediment under nitrate-reducing conditions. The degradation rates of benzene, toluene, ethylbenzene, and xylene by strain MAH1 were 4.71, 6.59, 5.64, and 2.59 mg x [L.sup.-1] x [day.sup.-1], respectively. The effects of sodium citrate, nitrate, and Na[H.sub.2]P[O.sub.4] on improving BTEX biodegradation were investigated, and their optimum concentrations were 0.5 g x [L.sup.-1], 100 mg x [L.sup.-1], and 0.8 mmol x [L.sup.-1], respectively. Moreover, MAH1, which has nirS and nosZgenes, removed ammonium, nitrate, and nitrite at 2.49 mg N[H.sub.4.sup.+]-N x [L.sup.-1] x [h.sup.-1], 1.50 mg N[O.sub.3.sup.-]-N x [L.sup.-1] x [h.sup.-1], and 0.83 mg N[O.sup.2.sup.-]-N x [L.sup.-1] x [h.sup.-1], respectively. MAH1 could help in mitigating the pollution caused by nitrogen amendments for biostimulation. This study highlighted the feasibility of using MAH1 for the bioremediation of BTEX-contaminated sites. Key words: BTEX, biodegradation, bioremediation, nitrogen removal, Pseudomonas thivervalensis MAH1. Le benzene, le toluene, l'ethylbenzene et le xylene (BTEX) representent un danger pour l'environnement en raison de leur presence repandue dans l'eau souterraine et le sol, ce qui fait peser une menace grandissante sur la sante humaine. Pseudomonas thivervalensis MAH1 est une bacterie aerobie denitrifiante degradant les BTEX qu'on a isolee d'un milieu contamine aux BTEX dans des conditions de reduction de nitrate. Les vitesses de degradation du benzene, du toluene, de l'ethylbenzene et du xylene par MAH1 etaient de 4,71, 6,59, 5,64 et 2,59 mg x [L.sup.-1] [jour.sup.-1], respectivement. On a examine l'influence positive du citrate de sodium, du nitrate et du Na[H.sub.2]P[O.sub.4] sur la biodegradation des BTEX; les concentrations optimales etaient respectivement de 0,5 g x [L.sup.-1], 100 mg x [L.sup.-1] et 0,8 mmol x [L.sup.-1], respectivement. Par ailleurs, MAH1 (qui renferme les genes nirS et nosZ) a elimine l'ammonium, le nitrate et le nitrite a des vitesses respectives de 2,49 mg N[H.sub.4.sup.+]-N x [L.sup.-1] x [h.sup.-1],1,50 mg N[O.sub.3.sup.- ]-N x [L.sup.-1] x [h.sup.-1] et 0,83 mg N[O.sub.2.sup.-]-N x [L.sup.-1] x [h.sup.-1]. MAH1 pourrait contribuer a endiguer la pollution causee par les supplementations d'azote aux fins de biostimulation. La presente etude met en evidence la faisabilite de l'utilisation de MAH1 pour la biorestauration de sites contamines aux BTEX. [Traduit par la Redaction] Mots-cles : BTEX, biodegradation, biorestauration, elimination de l'azote, Pseudomonas thivervalensis MAH1.

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Source Citation   (MLA 8th Edition)
Qu, Dan, et al. "BTEX biodegradation and its nitrogen removal potential by a newly isolated Pseudomonas thivervalensis MAH1." Canadian Journal of Microbiology, vol. 61, no. 9, 2015, p. 691+. Gale Academic Onefile, Accessed 7 Dec. 2019.

Gale Document Number: GALE|A436995112