Isolation and identification of microorganisms present in 53 dogs suffering otitis externa

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Publisher: Universidad del Zulia, Facultad de Ciencias Veterinarias
Document Type: Article
Length: 3,912 words
Lexile Measure: 1650L

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Aislamiento e identificacion de microorganismos presentes en 53 perros enfermos de otitis externa

ABSTRACT

Otitis externa is a multi-factorial etiology disease commonly affecting canines. It represents a complex pathology associated to infections caused by bacteria and yeasts, many times not responding to treatments. The goal of this study was to isolate and identify the pathogenic and non-pathogenic micro-organisms existent in otitis externa patients. This research was performed on 53 canine patients which were attended at the Outpatient Consult Service of the University Veterinary Policlinic at Zulia State University (UVP), and which showed clinical signs such as: auditory canal erythema, increased secretion, pain, continuous head shaking and pruritus, purulent secretions and pruritus being the most frequent. Samples were recollected from auditory canal horizontal portion exudates. Different bacteria groups were isolated in culture media, the most frequent were Pseudomona aeruginosa (22.22%), Proteus mirabilis (13.89%), Staphylococcus aureus (12.50%), Staphylococcus epidermidis (8.33%), Escherichia coli (5.56%) and Staphylococcus coagulase-negative (5.58%). In the control group, Bacillus spp. (16.67%), Staphylococcus epidermidis (16.67%) and Staphylococcus coagulase-negative (16.67%) were the most frequently isolated. Cytological examination showed Malassezia pachydermatis to be present in 69.8% of the cases studied. The most affected breeds were Poodles (30.19%), Mongrels (26.42%), Cocker Spaniels (16.98%) and German Shepherd Dogs (9.49%); likewise, a higher incidence of otitis was evidenced in dogs between 2 and 5 months of age (43.40%).

Key words: Otitis externa, canines, isolation, bacteria, yeasts.

RESUMEN

La otitis externa es una enfermedad de etiologia multifactorial que comunmente afecta a los caninos. Representa una patologia compleja que se asocia a infecciones causadas por bacterias y levaduras, y que muchas veces no responden a los tratamientos. El objetivo de este estudio fue aislar e identificar los microorganismos patogenos y no patogenos presentes en pacientes con otitis externa. Esta investigacion fue realizada sobre 53 pacientes caninos con otitis externa que fueron atendidos en el Servicio de Consulta Externa de la Policlinica Veterinaria de La Universidad del Zulia, los mismos presentaron signos clinicos tales como: eritema del canal auditivo, aumento de las secreciones, dolor, sacudidas constantes de la cabeza y prurito, siendo las secreciones purulentas y el prurito los mas resaltantes. Las muestres fueron colectadas de los exudados presentes en la porcion horizontal del canal auditivo. Diferentes grupos de bacterias fueron aisladas en medios de cultivos, presentandose con mayor frecuencia Pseudomona aeruginosa (22,22%), Proteus rnirabi/is (13,89%), Staphylococcus aureus (12,50%), Staphylococcus epidermidis (8,33%), Escherichia coli (5,56%) y Staphylococcus coagulasa negativa (5,56%). Por medio de la evaluacion citologica se evidencio que Malassezia pachydermatis se encontro presente en un 69,80% de los casos estudiados. Las razas mayormente afectadas fueron Poodle (30,19%), mestizos (26,42%), Cockers spaniel (16,98%) y Pastor aleman (9,49%); asi mismo se observo mayor incidencia de otitis en caninos entre los 2 y 5 meses de edad (43,40%).

Palabras clave: Otitis externa, caninos, aislamiento, bacterias, levaduras.

INTRODUCTION

Otitis externa has been reported in canines in a proportion varying from 5% to 20% [1, 9, 25], though in tropical climates prevalence is more near 30% to 40% [1, 25]. Otitis externa...

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Gale Document Number: GALE|A152291674