Novel reassortant influenza A(H5N8) viruses, South Korea, 2014

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From: Emerging Infectious Diseases(Vol. 20, Issue 6)
Publisher: U.S. National Center for Infectious Diseases
Document Type: Letter to the editor
Length: 1,242 words
Lexile Measure: 1530L

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To the Editor: Highly pathogenic avian influenza (HPAI) viruses have caused considerable economic losses to the poultry industry and poses potential threats to animal and human health (www.oie.int/en/ and www.who.int/en/). Since 2003, influenza A(H5N1) viruses with a hemagglutinin (HA) gene derived from A/goose/Guandong/1/96-like viruses have become endemic to 6 countries (Bangladesh, China, Egypt, India, Indonesia, and Vietnam) (1) (www.cdc. gov/). Furthermore, HPAI viruses with an H5 subtype continue to undergo substantial evolution because of extensive genetic divergence and reassortment between other subtypes of influenza viruses. Especially in China, novel subtypes of H5 HPAI virus, such as influenza A(H5N2), influenza A(H5N5), and influenza A(H5N8) viruses, were reported during 2009-2011 (2,3).

On January 16, 2014, clinical signs of HPAI, such as decreased egg production (60%) and slightly increased mortality rates, were detected in ducks on a breeder duck farm near the Donglim Reservoir in Jeonbuk Province, South Korea. On January 17, a farmer (5 km from the Donglim Reservoir) also reported clinical signs of HPAI in broiler ducks. In addition, 100 carcasses of Baikal teals were found in the Donglim Reservoir.

RNAs extracted from organs (liver, pancreas, and trachea) of 3 dead birds (1 breeder duck, 1 broiler duck, and 1 Baikal teal) were positive for H5 subtype virus by reverse transcription PCR (4). We isolated viruses from suspected specimens by inoculation into embryonated specific pathogen-free chicken eggs. The H5N8 subtype was identified by using HA and neuraminidase (NA) inhibition assays.

Three viruses isolated from domestic ducks and wild birds were designated A/breeder duck/Korea/ Gochang1/2014 (H5N8) (Gochang1), A/duck/Korea/Buan2/2014 (H5N8) (Buan2), and A/Baikal Teal/Korea/ Donglim3/2014 (H5N8) (Donglim3).

All 8 RNA genome segments of these viruses were amplified by using segment-specific primers and directly sequenced (5). Sequences of the 8 RNA segments of each virus were submitted to GenBank under accession nos. KJ413831-KJ413854.

Gochang1 virus has been shown to be highly pathogenic for chickens...

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Gale Document Number: GALE|A372956955