Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry/Nova metoda detekcie CRP proteinu na kralicich leukocytoch pomocou prietokovej cytometrie

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From: Journal of Central European Agriculture(Vol. 20, Issue 1)
Publisher: Sveuciliste U Zagrebu
Document Type: Article
Length: 2,845 words
Lexile Measure: 1400L

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Abstract :

C-reactive protein (CRP) is an acute phase serum protein secreted by liver hepatocytes. Besides its presence in the serum, CRP was also found on the surface of human leukocytes. However, the binding ability of CRP to rabbit leukocytes has never been previously investigated. The objective of this study was to optimize the detection of rabbit CRP binding leukocytes in order to observe the acute phase of immune response by flow cytometry after Complete Freund's Adjuvant (CFA) administration. Blood samples were analysed using ELISA assay and flow cytometry immediately before and 2 days after the CFA administration. Significant (P Keywords: C-reactive protein, Complete Freund's adjuvant, ELISA, flow cytometry, rabbit leukocytes C-reaktivny protein (CRP) je serovy protein akutnej fazy vylucovany pecehovymi hepatocytmi. Popri jeho vyskyte v sere bolo zistene, ze CRP sa nachadza aj na povrchu l'udskych leukocytov. Avsak, schopnost' CRP viazat' sa na kralicie leukocyty, nebola doteraz preskumana. Ciel'om nasej studie bolo optimalizovat' detekciu kralicich leukocytov viazucich CRP pomocou prietokovej cytometrie za ucelom sledovania akutnej fazy imunitnej odpovede vyvolanej aplikaciou Kompletneho Freundovho adjuvansu (CFA). Vzorky krvi boli analyzovane pomocou ELISA metody a prietokovou cytometriou ihned' pred a 2 dni po aplikacii CFA. Vo vzorkach po CFA imunizacii bol zaznamenany signifikantny (P Kl'ucove slova: C-reaktivny protein, ELISA, Kompletny Freundov adjuvans, kralicie leukocyty, prietokova cytometria

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Gale Document Number: GALE|A583249711