Search Results

• • 163 

    Academic Journals

  • Sort by: sorttype.publication.date.desc Newest sorttype.publication.date.asc Oldest sorttype.relevance Relevance

• Polymerase chain reaction optimization for amplification of Guanine-Cytosine rich templates using buccal cell DNA

  Authors:C. H W M R. Bhagya, W. Wijesundera Sulochana, and N. HemamaliFrom:Indian Journal of Human Genetics (Vol. 19, Issue 1) Peer-Reviewed

  January-March 20133,473 words Report 1630LByline: C. H. W. M. R.. Bhagya, W. Wijesundera Sulochana, N. Hemamali Context: Amplification of Guanine-Cytosine (GC) -rich sequences becomes important in screening and diagnosis of certain genetic diseases such as...

• RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts

  Authors:Joelle Vermeulen, Stefaan Derveaux, Steve Lefever, Els De Smet, Katleen De Preter, Nurten Yigit, et al.From:BMC Research Notes (Vol. 2) Peer-Reviewed

  Nov. 25, 20094,121 words Report Authors: Joëlle Vermeulen (equal contributor) [1]; Stefaan Derveaux (equal contributor) [1]; Steve Lefever [1]; Els De Smet [1]; Katleen De Preter [1]; Nurten Yigit [1]; Anne De Paepe [1]; Filip Pattyn [1]; Frank...

• Amplification of bacterial DNA using highly conserved sequences: automated analysis and potential for molecular triage of sepsis

  Authors:Kevin M. McCabe, Ghazala Khan, Yao-Hua Zhang, Edward O. Mason, and Edward R.B. McCabeFrom:Pediatrics (Vol. 95, Issue 2) Peer-Reviewed

  Feb. 19953,608 words Article Quick diagnosis of the presence of sepsis in infants and young children may be made possible by a new automated technique that amplifies DNA sequences of bacteria in blood and other body fluids. Sepsis is characterized...

• ClampFISH detects individual nucleic acid molecules using click chemistry-based amplification

  Authors:Sara H Rouhanifard, Ian A Mellis, Margaret Dunagin, Sareh Bayatpour, Connie L Jiang, Ian Dardani, et al.From:Nature Biotechnology (Vol. 37, Issue 1) Peer-Reviewed

  Jan. 20197,432 words Report 1580LAuthor(s): Sara H Rouhanifard [1]; Ian A Mellis [1, 2]; Margaret Dunagin [1]; Sareh Bayatpour [1]; Connie L Jiang [1, 3]; Ian Dardani [1]; Orsolya Symmons [1]; Benjamin Emert [1, 2]; Eduardo Torre [1, 4]; Allison Cote...

• Development of loop-mediated isothermal amplification for detection of Leifsonia xyli subsp. xyli in sugarcane

  Authors:Jing Liu, Liping Xu, Jinlong Guo, Rukai Chen, Michael Paul Grisham, and Youxiong QueFrom:BioMed Research InternationalPeer-Reviewed

  Annual 20134,602 words Report 1520LRatoon stunt, caused by the xylem-limited coryneform bacterium Leifsonia xyli subsp. xyli (Lxx), is a deep bacteriosis and prevalent in most of sugarcane-producing countries. Based on loop-mediated isothermal...

• Diagnostic accuracy of the loop-mediated isothermal amplification assay for extrapulmonary tuberculosis: A meta-analysis

  Authors:Guocan Yu, Yanqin Shen, Fangming Zhong, Bo Ye, Jun Yang, and Gang ChenFrom:PLoS ONE (Vol. 13, Issue 6) Peer-Reviewed

  June 26, 20185,130 words Report 1420LBackground Loop-mediated isothermal amplification (LAMP) is used to detect pulmonary tuberculosis (PTB); however, the diagnostic accuracy of the LAMP assay for extrapulmonary tuberculosis (EPTB) is unclear. We...

• Rapid SARS-CoV-2 testing in primary material based on a novel multiplex RT-LAMP assay

  Authors:Bernhard Schermer, Francesca Fabretti, Maximilian Damagnez, Veronica Di Cristanziano, Eva Heger, Sita Arjune, et al.From:PLoS ONE (Vol. 15, Issue 11) Peer-Reviewed

  Nov. 2, 20205,791 words Report 1450LBackground Rapid and extensive testing of large parts of the population and specific subgroups is crucial for proper management of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections and...

• Overexpression of Ad5 precursor terminal protein accelerates recombinant adenovirus packaging and amplification in HEK-293 packaging cells

  Authors:N. Wu, H. Zhang, F. Deng, R. Li, W. Zhang, X. Chen, et al.From:Gene Therapy (Vol. 21, Issue 7) Peer-Reviewed

  July 20145,104 words Report 1830LRecombinant adenoviruses are one of the most common vehicles for efficient in vitro and in vivo gene deliveries. Here, we investigate whether exogenous precursor terminal protein (pTP) expression in 293 cells improves...

• Detection and quantification of the nifH gene in shoot and root of cucumber plants

  Authors:Dilafruz Juraeva, Eckhard George, Kahramon Davranov, and Silke RuppelFrom:Canadian Journal of Microbiology (Vol. 52, Issue 8) Peer-Reviewed

  Aug. 20065,836 words Article 1840LAbstract: A real-time polymerase chain reaction (PCR) method was applied to quantify the nifH gene pool in cucumber shoot and root and to evaluate how nitrogen (N) supply and plant age affect the nifH gene pool. In...

• LAMPS: an analysis pipeline for sequence-specific ligation-mediated amplification reads

  Authors:Christopher J. F. Cameron, Xue Q. D. Wang, Josee Dostie, and Mathieu BlanchetteFrom:BMC Research Notes (Vol. 13, Issue 1) Peer-Reviewed

  June 3, 20201,505 words Report 1570LAuthor(s): Christopher J. F. Cameron1,2 , Xue Q. D. Wang 2 , Josée Dostie2 and Mathieu Blanchette1 Introduction Ligation-Mediated Amplification (LMA) library preparation protocols [1, 2] have become increasingly...

• Genomic amplification and high expression of EGFR are key targetable oncogenic events in malignant peripheral nerve sheath tumor

  Authors:Xiaoling Du, Jilong Yang, Antti Ylipaa, and Ze ZhuFrom:Journal of Hematology & Oncology (Vol. 6, Issue 1) Peer-Reviewed

  Dec. 17, 20136,496 words Report 1540LBackground The dismal outcome of malignant peripheral nerve sheath tumor (MPNST) highlights the necessity of finding new therapeutic methods to benefit patients with this aggressive sarcoma. Our purpose was to...

• Improvement and Evaluation of Loop-Mediated Isothermal Amplification for Rapid Detection of Toxoplasma gondii Infection in Human Blood Samples

  Authors:Xi-meng Sun, Yong-sheng Ji, Xian-yong Liu, Mei Xiang, Guang He, Li Xie, et al.From:PLoS ONE (Vol. 12, Issue 1) Peer-Reviewed

  Jan. 5, 20174,652 words Report 1440LLoop-mediated isothermal amplification (LAMP), an attractive DNA amplification method, was developed as a valuable tool for the rapid detection of Toxoplasma gondii. In this study, species-specific LAMP primers were...

• Simultaneous rapid detection of Hantaan virus and Seoul virus using RT-LAMP in rats

  Authors:Xin Sui, Xu Zhang, Dongliang Fei, Zhen Zhang, and Mingxiao MaFrom:PeerJ (Vol. 7) Peer-Reviewed

  Jan. 8, 20195,049 words Report 1500LBackground Hemorrhagic fever with renal syndrome is in most cases caused by the Hantaan virus (HTNV) and Seoul virus (SEOV). To develop and apply reverse transcription loop-mediated isothermal amplification (RT-LAMP)...

• Rapid molecular diagnosis of Parechovirus infection using the reverse transcription loop-mediated isothermal amplification technique.

  Authors:Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, et al.From:PLoS ONE (Vol. 16, Issue 11) Peer-Reviewed

  Nov. 29, 20213,648 words Report 1270LObjectives Human parechovirus (HPeV), especially HPeV A3 (HPeV3), causes sepsis-like diseases and sudden infant death syndrome in neonates and young infants. Development of rapid and easier diagnostic laboratory tests...

• Escape from adaptive conflict after duplication in an anthocyanin pathway gene

  Authors:David L. Des Marais and Mark D. RausherFrom:Nature (Vol. 454, Issue 7205) Peer-Reviewed

  Aug. 7, 20084,558 words Clinical report 1470LGene duplications have been recognized as an important source of evolutionary innovation and adaptation since at least Haldane (1), and their varying fates may partly explain the vast disparity in observed genome sizes...

• A new treasure in the breast cancer gene hunt

  Authors:Paul Spellman and Joe GrayFrom:Nature Medicine (Vol. 17, Issue 4) Peer-Reviewed

  Apr. 20111,425 words Report 1560LA new potential breast cancer oncogene, ZNF703, has been identified in the chromosomal region 8p12 in humans, which is commonly amplified in an aggressive subtype of breast cancer. ZNF703 is a transcriptional repressor...

• Ante- and post-mortem diagnosis of rabies using nucleic acid-amplification tests

  Authors:Supaporn Wacharapluesadee and Thiravat HemachudhaFrom:Expert Review of Molecular Diagnostics (Vol. 10, Issue 2) Peer-Reviewed

  Mar. 20109,652 words Report Author(s): Supaporn Wacharapluesadee [[dagger]] 1 , Thiravat Hemachudha 2 Keywords: ante-mortem; detection; diagnosis; nucleic acid amplification; PCR; post-mortem; rabies; real-time PCR Rabies remains a...

• Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates

  Authors:Takeshi Sano, Cassandra L. Smith, and Charles R. CantorFrom:Science (Vol. 258, Issue 5079) Peer-Reviewed

  Oct. 2, 19922,736 words Article An antigen detection system, termed immuno-polymerase chain reaction (immuno-PCR), was developed in which a specific DNA molecule is used as the marker. A streptavidin-protein A chimera that possesses tight and specific...

• erbB-2 is a potent oncogene when overexpressed in NIH-3T3 cells

  Authors:Pier Paolo di Fiore, Jacalyn H. Pierce, Matthias H. Kraus, Oreste Segatto, C. Richter King, and Stuart A. AaronsonFrom:Science (Vol. 237) Peer-Reviewed

  July 10, 19873,663 words Article erbB-2 Is a Potent Oncogene When Overexpressed in NIH/3T3 Cells EVIDENCE THAT RETROVIRAL ONCOGENEScan encode proteins that are homologous to either growth factors or growth factor receptors has shed new light on the...

• Application of loop-mediated isothermal amplification assay in the detection of herpesvirus of turkey (FC 126 strain) from chicken samples in Nigeria

  Authors:A.J. Adedeji, P.A. Abdu, P.D. Luka, A.A. Owoade, and T.M. JoannisFrom:Veterinary World (Vol. 10, Issue 11)

  Nov. 20173,961 words Report 1500LAim: This study was designed to optimize and apply the use of loop-mediated isothermal amplification (LAMP) as an alternative to conventional polymerase chain reaction (PCR) for the detection of herpesvirus of turkeys...