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• Glossary of HPLC and LC separation terms

  Authors:Ronald E. Majors and Peter W. CarrFrom:LC-GC North America (Vol. 26, Issue 2) Peer-Reviewed

  Feb. 200819,463 words Article 1030LIn this installment of "Column Watch," columnist Ron Majors and guest coauthor, Prof. Peter Carr of the University of Minnesota update the popular HPLC-LC glossary that covers common and not-so-common terms used in...

• Protein quantification by derivatization-free high-performance liquid chromatography of aromatic amino acids

  Authors:Almut Hesse and Michael G. WellerFrom:Journal of Amino AcidsPeer-Reviewed

  Annual 20165,886 words Report 1400LAmino acid analysis is considered to be the gold standard for quantitative peptide and protein analysis. Here, we would like to propose a simple HPLC/UV method based on a reversed-phase separation of the aromatic amino...

• Chiralizer Services, LLC: www.hplctools.com. (Web Update)

  From:LC-GC North America (Vol. 20, Issue 3) Peer-Reviewed

  Mar. 200277 words Brief article Chiralizer Services' (Newtown, Pennsylvania) web site features HPLC, LC-MS, and sample preparation products available from the company. The site includes charts and tables with information about HPLC column volumes,...

• Drip, drip, drip ...: some things never change

  Author:John W. DolanFrom:LC-GC North America (Vol. 26, Issue 10) Peer-Reviewed

  Oct. 20082,181 words Column This "LC Troubleshooting" installment marks the beginning of the 26th year that I have been writing this column. Over that time, changes in instrument design, and especially column technology, have occurred that have...

• Approaches to singleton achiral purification of difficult samples for discovery research support in the pharmaceutical industry

  Authors:Tony Q. Yan, Jim Bradow, Shang-Poa Chang, Rob Depianta, and Laurence PhilippeFrom:LC-GC North America (Vol. 27, Issue 4) Peer-Reviewed

  Apr. 20092,613 words Column Several approaches for purifying difficult samples more efficiently for discovery research support are mentioned in this paper. These approaches use mass triggered high performance liquid chromatography (HPLC) on...

• Buffer considerations for LC and LC-MS

  Authors:Xavier Subirats, Elisabeth Bosch, and Marti RosesFrom:LC-GC North America (Vol. 27, Issue 11) Peer-Reviewed

  Nov. 20092,389 words Article In this article, the buffer capacity concept is revisited, particularly concerning its behavior in hydroorganic mobile phases. The buffer capacity of a polyprotic acid, or a mixture of monoprotic acids, depends upon the...

• Gas chromatography determination of trace of biomarkers of mustard agent and some organophosphoric toxic agents in urine

  Authors:I.N. Stan'kov, V.B. Kondrat'ev, I.D. Derevyagina, O.T. Morozova, S.V. Sadovnikov, V.I. Selivanova, et al.From:Journal of Analytical Chemistry (Vol. 66, Issue 7) Peer-Reviewed

  July 20112,342 words Article 1380LA procedure has been developed for the determination of thioglycol, 1,1'-sulfonyl-bis[2-(methylsulfonyl)-ethane], 1-methylsulfonyl-2-[2-(methylthio)ethylsulfonyl]ethane (biomarkers of mustard agent) and methylphosphonic...

• Quantitative open-access HPLC analysis: a new calibration approach: a novel tool enables users to generate reliable data for yields in solution and for assays of isolated solids using high-performance liquid chromatography without having to prepare standards. The authors discuss the approach taken to develop the tool, its associated protocols and how it can be used to calculate data

  Authors:Phil Borman, John Roberts, Barbara O'Reilly, Robin Attrill, Ian Barylski, and Keith FreebairnFrom:Pharmaceutical Technology Europe (Vol. 22, Issue 10) Peer-Reviewed

  Oct. 20102,630 words Article 1550LThe use of open-access high-performance liquid chromatography (HPLC) systems in drug discovery and pharmaceutical development has steadily increased during the past decade. This growth has enabled chemists and engineers...

• Technologies vie for dominance: liquid chromatography gains adherents, but 2-D gel-based proteomics sallies forth. (Proteomics)

  Author:Jeffrey M. PerkelFrom:The Scientist (Vol. 17, Issue 5) Peer-Reviewed

  Mar. 10, 20032,162 words Article Thierry Rabilloud has been doing proteomics since long before the word proteome was even coined. For years Rabilloud, currently at the Atomic Energy Commission Research Center in Grenoble, France, used two-dimensional...

• Isolation of a G protein that is modified by learning and reduces potassium currents in Hermissenda

  Authors:Thomas J. Nelson, Carlos Collin, and Daniel L. AlkonFrom:Science (Vol. 247, Issue 4949) Peer-Reviewed

  Mar. 23, 19902,481 words Article Isolation of a G Protein That Is Modified by Learning and Reduces Potassium Currents in Hermissenda IN THE TYPE B PHOTORECEPTOR NEUrons in the eye, associative conditioning of the marine nudibranch mollusk Hermissenda...

• Posttranslational glutamylation of alpha-tubulin

  Authors:Bernard Edde, Jean Rossier, Jean-Pierre Le Caer, Elizabeth Desbruyeres, Francois Gros, and Philippe DenouletFrom:Science (Vol. 247, Issue 4938) Peer-Reviewed

  Jan. 5, 19901,835 words Article Posttranslational Glutamylation of [alpha]-Tubulin TUBULIN HETEROGENEITY IS PARTICULARLY high in the brain where up to 21 variants can be resolved by isoelectric focusing (IEF) techniques [1, 2]. In the mouse, five...

• CSF Concentrations of cAMP and cGMP Are Lower in Patients with Creutzfeldt-Jakob Disease but Not Parkinson's Disease and Amyotrophic Lateral Sclerosis

  Authors:Patrick Oeckl, Petra Steinacker, Stefan Lehnert, Sarah Jesse, Hans A. Kretzschmar, Albert C. Ludolph, et al.From:PLoS ONE (Vol. 7, Issue 3) Peer-Reviewed

  Mar. 2, 20125,778 words Article 1340LAuthor(s): Patrick Oeckl 1 , Petra Steinacker 2 , Stefan Lehnert 2 , Sarah Jesse 2 , Hans A. Kretzschmar 3 , Albert C. Ludolph 2 , Markus Otto 2 , Boris Ferger 1 , * Introduction The cyclic nucleotides cyclic...

• Proteomic Analysis Reveals Differentially Regulated Protein Acetylation in Human Amyotrophic Lateral Sclerosis Spinal Cord

  Authors:Dong Liu, Chaoxu Liu, Junqiang Li, Kazem Azadzoi, Yun Yang, Zhou Fei, et al.From:PLoS ONE (Vol. 8, Issue 12) Peer-Reviewed

  Dec. 2, 20136,708 words Article 1460LAuthor(s): Dong Liu 1,2, Chaoxu Liu 1,2, Junqiang Li 3, Kazem Azadzoi 2, Yun Yang 2, Zhou Fei 1, Kefeng Dou 1, Neil W. Kowall 2, Han-Pil Choi 4, Fernando Vieira 5, Jing-Hua Yang 2,3,4,* Introduction Amyotrophic...

• Transient Exposure to Low Levels of Insecticide Affects Metabolic Networks of Honeybee Larvae

  Authors:Kamila Derecka, Martin J. Blythe, Sunir Malla, Diane P. Genereux, Alessandro Guffanti, Paolo Pavan, et al.From:PLoS ONE (Vol. 8, Issue 7) Peer-Reviewed

  July 2, 20138,170 words Report 1410LAuthor(s): Kamila Derecka 1, Martin J. Blythe 2, Sunir Malla 2, Diane P. Genereux 3, Alessandro Guffanti 4, Paolo Pavan 4, Anna Moles 4, Charles Snart 1, Thomas Ryder 5, Catharine A. Ortori 6, David A. Barrett 6, Eugene...

• Evidence and quantitation of aromatic organosulfates in ambient aerosols in Lahore, Pakistan

  Authors:S. Kundu, T. A. Quraishi, G. Yu, C. Suarez, F. N. Keutsch, and E. A. StoneFrom:Atmospheric Chemistry and Physics (Vol. 13, Issue 9) Peer-Reviewed

  May 14, 201336 words Brief article Byline: S. Kundu, T. A. Quraishi, G. Yu, C. Suarez, F. N. Keutsch, E. A. Stone To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link:...

• Microcystin-Degrading Activity of an Indigenous Bacterial Strain Stenotrophomonas acidaminiphila MC-LTH2 Isolated from Lake Taihu

  Authors:Fei Yang, Yuanlong Zhou, Lihong Yin, Guangcan Zhu, Geyu Liang, and Yuepu PuFrom:PLoS ONE (Vol. 9, Issue 1) Peer-Reviewed

  Jan. 9, 20144,318 words Article 1470LAuthor(s): Fei Yang, Yuanlong Zhou, Lihong Yin, Guangcan Zhu, Geyu Liang, Yuepu Pu * Introduction Harmful cyanobacterial blooms (HCBs) have occurred frequently in eutrophic lakes all over the world in the past two...

• Nutritional Profile of Phytococktail from Trans-Himalayan Plants

  Authors:Priyanka Dhar, Amol B. Tayade, Jatinder Kumar, Om P. Chaurasia, Ravi B. Srivastava, and Shashi B. SinghFrom:PLoS ONE (Vol. 8, Issue 12) Peer-Reviewed

  Dec. 23, 201312,885 words Article 1480LAuthor(s): Priyanka Dhar 1, Amol B. Tayade 1, Jatinder Kumar 1, Om P. Chaurasia 1,*, Ravi B. Srivastava 1, Shashi B. Singh 2 Introduction Plant-derived foods such as fruits, vegetables, grains, oils, nuts etc....

• Condensed Tannins from Ficus virens as Tyrosinase Inhibitors: Structure, Inhibitory Activity and Molecular Mechanism

  Authors:Xiao-Xin Chen, Yan Shi, Wei-Ming Chai, Hui-Ling Feng, Jiang-Xing Zhuang, and Qing-Xi ChenFrom:PLoS ONE (Vol. 9, Issue 3) Peer-Reviewed

  Mar. 17, 20146,836 words Article 1550LAuthor(s): Xiao-Xin Chen 1, Yan Shi 1, Wei-Ming Chai 1, Hui-Ling Feng 1, Jiang-Xing Zhuang 2,*, Qing-Xi Chen 1,* Introduction Tyrosinase (EC 1.14.18.1) is a copper-containing, mixed-function oxidase widely...

• Association of the Plasma and Tissue Riboflavin Levels with C20orf54 Expression in Cervical Lesions and Its Relationship to HPV16 Infection

  Authors:Aixingzi Aili, Ayshamgul Hasim, Alimujiang Kelimu, Xia Guo, Batur Mamtimin, Abuliz Abudula, et al.From:PLoS ONE (Vol. 8, Issue 11) Peer-Reviewed

  Nov. 18, 20136,211 words Article 1470LAuthor(s): Aixingzi Aili 1, Ayshamgul Hasim 2, Alimujiang Kelimu 3, Xia Guo 4, Batur Mamtimin 5, Abuliz Abudula 4, Halmurat Upur 4,* Introduction Worldwide, cervical cancer has the second highest incidence of...

• Identification of Ochratoxin A Producing Fungi Associated with Fresh and Dry Liquorice

  From:PLoS ONE (Vol. 8, Issue 10) Peer-Reviewed

  Oct. 21, 20135,567 words Article 1390LIntroduction Liquorice, the root of the leguminous Glycyrrhiza plant species (Glycyrrhiza uralensis Fisch., Glycyrrhiza inflate Bat. and Glycyrrhiza glabra L.), is a popular botanical with a long history of...