Impact of dietary fiber/starch ratio in shaping caecal microbiota in rabbits

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Date: Oct. 2015
From: Canadian Journal of Microbiology(Vol. 61, Issue 10)
Publisher: NRC Research Press
Document Type: Report
Length: 7,031 words
Lexile Measure: 1480L

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Abstract :

The objective of this experiment was to determine whether changing the dietary neutral detergent fiber (NDF)/starch ratio affected caecal microbiota when 4 different diets (diet A: 2.3 NDF/starch, diet B: 1.9, diet C: 1.4, diet D: 1.0) were formulated. A total of 200 weaned rabbits (35 days old, 50 per group) were used for the experiment, which started after an adaptation period of 7 days (i.e., day 42). Caecal contents were obtained from rabbits fed different NDF/starch diets at 52, 62, 72, and 82 days of life. The bacterial community structure was characterized by high-throughput 16S rRNA sequencing. Firmicutes, Actinobacteria, Synergistetes, and Tenericutes did not significantly change with diet or age. However, Bacteroidetes (P Key words: rabbit, fiber/starch, caecum, microbiota. La presente experience avait pour but de determiner si le rapport entre les fibres insolubles au detergent neutre (FDN) et l'amidon dans l'alimentation affectait la microflore caecale lorsque quatre regimes differents etaient formules (regime A : 2,3; regime B : 1,9; regime C : 1,4; regime D : 1,0). Un ensemble de 200 lapins sevres (35 jours de vie, 50 par groupe) ont ete utilises pour l'experience, qui a demarre apres une periode d'adaptation de 7 jours, soit au jour 42. Le contenu des caecums a ete preleve de lapins nourris avec divers regimes de FDN/amidon aux jours 52,62,72 et 82 de leur vie, respectivement. On a caracterise la structure communautaire des bacteries par sequencage a haut debit de l'ARNr 16S. On n'a remarque aucune variation significative des Firmicutes, Actinobacteria, Synergistetes ou Tenericutes selon l'alimentation ou l'age. Cependant, les lectures de Bacteroidetes (P Mots-cles: lapin, fibres/amidon, caecum, microflore.

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Gale Document Number: GALE|A430494119