Screen-less expanded bed column: new approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris.

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From: Biotechnology Letters(Vol. 28, Issue 13)
Publisher: Springer
Document Type: Report; Brief article
Length: 203 words

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Abstract :

Keywords: Expanded bed adsorption; Malaria vaccine; Pichia pastoris Abstract An experimental malaria transmission blocking vaccine antigen, Pfs25H, expressed and secreted from Pichia pastoris was recovered and purified using a screenless expanded bed column equipped with a rotating fluid distribution system. This column was able to accommodate feed stock, containing 30% biomass, at a flow rate of 300--400 cm/h without affecting column stability. This capability is three times higher than the capability of the expanded bed column currently in use, which is equipped with a perforated plate fluid distribution system this design could accommodate biomass concentrations of only up to 10%. The screen-less design did not affect the binding capacity, purification level or process yield and, therefore, shorten the process. Purified Pfs25H of 6.4 g were recovered from 37 l of Pichia pastoris culture in one step. Author Affiliation: (1) Biotechnology Unit, NIDDK, NIH Bldg 14A, Room 173, 20892, Bethesda, MD, USA (2) Malaria Vaccine Development Branch, NIAID, 20852, NIH Rockville, MD , USA (f) Article History: Registration Date: 03/30/2006 Received Date: 01/06/2006 Accepted Date: 02/28/2006 Online Date: 06/20/2006 Byline: Loc Trinh (1), Je-Nie Phue (1), Pratik Jaluria (1), Chiawei W. Tsai (2), David L. Narum (2), Joseph Shiloach (corresponding author) (1, f)

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Gale Document Number: GALE|A628005727