Morphological study of embryonic Chd8+/- mouse brains using light-sheet microscopy.

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Date: Jan. 12, 2021
From: BMC Research Notes(Vol. 14, Issue 1)
Publisher: BioMed Central Ltd.
Document Type: Report
Length: 3,182 words
Lexile Measure: 1540L

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Abstract :

Objective Autism spectrum disorder (ASD) encompasses a group of neurodevelopmental conditions that remain poorly understood due to their genetic complexity. CHD8 is a risk allele strongly associated with ASD, and heterozygous Chd8 loss-of-function mice have been reported to exhibit macrocephaly in early postnatal stages. In this work, we sought to identify measurable brain alterations in early embryonic development. Results We performed light-sheet fluorescence microscopy imaging of N-cadherin stained and optically cleared Chd8.sup.+/- and wild-type mouse brains at embryonic day 12.5 (E12.5). We report a detailed morphometric characterization of embryonic brain shapes and cortical neuroepithelial apical architecture. While Chd8.sup.+/- characteristic expansion of the forebrain and midbrain was not observed this early in embryogenesis, a tendency for a decreased lateral ventricular sphericity and an increased intraocular distance in Chd8.sup.+/- brains was found compared to controls. This study advocates the use of high-resolution microscopy technologies and multi-scale morphometric analyses of target brain regions to explore the etiology and cellular basis of Chd8 haploinsufficiency. Keywords: Light-sheet microscopy, Chd8, Mouse brains, Autism, Tissue clearing, Morphometry, Lewis' law, Aboav-weaire's law

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Gale Document Number: GALE|A650605887