Spectroscopic and electrochemical approaches for the analysis of interaction between textile dye 231 and salmon sperm DNA.

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Date: Apr. 2022
From: Canadian Journal of Chemistry(Vol. 100, Issue 4)
Publisher: NRC Research Press
Document Type: Report
Length: 4,517 words
Lexile Measure: 1610L

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Abstract :

DNA is one of the most critical targets for many artificial agents listed as carcinogens. Most of them irreversibly bind to the DNA and induce genome mutation; therefore, it is vital to study the nature of binding of these molecules to anticipate their toxicity. The interaction between the textile dye reactive red 231 and salmon sperm double-stranded DNA (ss-dsDNA) was investigated by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and ultraviolet-visible spectroscopy (UV-vis spectroscopy). Changes in the anodic current signals of the dye were observed in the presence and absence of ss-dsDNA at a glassy carbon electrode (GCE) using CV. The diffusion coefficient (D) was found to be 2.2 * [10.sup.-7] and 9.5 * [10.sup.-8] [cm.sup.2] [s.sup.-1] from the CV data for the free dye and dye-DNA complex, respectively. Electrochemical and UV-vis spectroscopy indicated 1:1 complex formation of the dye with DNA. The binding constant (kb) between the dye and DNA was calculated to be 5.4 * [10.sup.5] [M.sup.-1] and 4.9 * [10.sup.5] [M.sup.-1] at pH 4.0 using CV and UV-vis spectroscopy, respectively. Overall, these results suggest that the dye binds to DNA through the combined effect of intercalation and electrostatic interactions. DNA damage was also detected through changes in the voltammetric behaviour of the dye. Key words: DNA binding, dye reactive red 231, UV-vis spectroscopy, voltammetry. LADN est l'une des plus importantes cibles de nombreux agents artificiels repertories comme cancerigenes. La plupart d'entre eux se lient de maniere irreversible a l 'ADN, induisant des mutations genomiques; il est donc essentiel d' etudier la nature de la liaison de ces molecules afin d'anticiper leur toxicite. Nous avons etudie l'interaction entre le colorant textile rouge reactif 231 et l'ADN double brin du sperme de saumon (ADNdbss) par voltamperometrie cyclique (VC), voltamperometrie differentielle pulsee (VDP) et spectroscopie UV-visible (UV-vis). Nous avons observe des variations entre les signaux de courant anodique du colorant, en presence et en absence dADNdbss, mesures par VC a une electrode de carbone vitreux (ECV). Nous avons obtenu des coefficients de diffusion (D) de 2,2 * [10.sup.-7] [cm.sup.2] [s.sup.-1] et de 9,5 * [10.sup.-8] [cm.sup.2] [s.sup.-1] a partir des donnees de VC pour le colorant libre et le complexe ADN-colorant, respectivement. Les methodes electrochimiques et la spectroscopie UV-vis indiquent la formation d'un complexe entre le colorant et l 'ADN dans une proportion de 1:1. Nous avons calcule des constantes de liaison ([k.sub.b]) entre le colorant et l'ADN de 5,4 * [10.sup.5] [M.sup.-1] et de 4,9 * [10.sup.5] [M.sup.-1] a pH 4,0 a l'aide respectivement de la VC et de la spectroscopie UV-vis. De maniere generale, les resultats laissent supposer que le colorant se lie a l'ADN par l'effet combine d'intercalation et d'interaction electrostatique. Nous avons egalement observe des changements dans le comportement voltamperometrique du colorant qui indiquent des dommages a l'ADN. [Traduit par la Redaction] Mots-cles: liaison de l'AND, colorant rouge reactif 231, spectroscopie UV-visible, voltamperometrie.

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Gale Document Number: GALE|A700235411