Microbial rRNA Synthesis and Growth Compared through Quantitative Stable Isotope Probing with H218O

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Date: Apr. 15, 2018
From: Applied and Environmental Microbiology(Vol. 84, Issue 8)
Publisher: American Society for Microbiology
Document Type: Article
Length: 88 words

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Abstract :

To elucidate whether metabolic activity of soil microorganisms is coupled with growth, the relationship between rRNA and DNA synthesis in a soil bacterial community is investigated using quantitative stable isotope probing (qSIP) with H218O. Most soil bacterial taxa are metabolically active and grow, and there is no significant difference between the isotopic composition of DNA and RNA extracted from soil incubated with H218O. The results support the notion that metabolic activity is strongly coupled to cellular division and suggest that nondividing taxa do not dominate soil metabolic activity.

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Gale Document Number: GALE|A542248026