The lack of BTK does not impair monocytes and polymorphonuclear cells functions in X-linked agammaglobulinemia under treatment with intravenous immunoglobulin replacement

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From: PLoS ONE(Vol. 12, Issue 4)
Publisher: Public Library of Science
Document Type: Report
Length: 8,990 words
Lexile Measure: 1410L

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Abstract :

The lack of BTK in X-linked agammaglobulinemia (XLA) patients does not affect monocytes and polymorphonuclear cells (PMN) phenotype and functions. In this study, we show that XLA patients had an increased frequency of the intermediate monocytes subset and that BTK-deficient monocytes and PMN had a normal expression of receptors involved in the activation and cellular responses. We demonstrate that BTK is not required for migration, phagocytosis and the production of reactive oxygen species (ROS) following engagement of FC gamma receptors (Fc[gamma]R). XLA monocytes and PMN showed an efficient calcium (Ca.sup.2+ )-independent activation of oxidative burst, suggesting that oxidative burst is less dependent by Ca.sup.2+ mobilization. The phagocytosis was functional and it remained unaltered also after Ca.sup.2+ chelation, confirming the independence of phagocytosis on Ca.sup.2+ mobilization. Intravenous immunoglobulin (IVIg) infusion exerted an anti-inflammatory effect by reducing the frequency of pro-inflammatory monocytes. In monocytes, the IVIg reduce the oxidative burst and phagocytosis even if these functions remained efficient.

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Gale Document Number: GALE|A490225422