Sex differences in ET-1 receptor expression and [Ca.sup.2+] signaling in the IMCD

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Publisher: American Physiological Society
Document Type: Author abstract; Report
Length: 300 words

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Abstract :

The inner medullary collecting duct (IMCD) is the nephron segment with the highest production of endothelin-1 (ET-1) and the greatest expression of ET-1 receptors that function to adjust [Na.sup.+] and water balance. We have reported that male rats have reduced natriuresis in response to direct intramedullary infusion of ET- 1 compared with female rats. Our aim was to determine whether alterations of ET-1 receptor expression and downstream intracellular [Ca.sup.2+] signaling within the IMCD could account for these sex differences. IMCDs from male and female rats were isolated for radioligand binding or microdissected for intracellular [Ca.sup.2+] ([[Ca.sup.2+]].sub.i]) measurement by fluorescence imaging of fura-2 AM. IMCD from male and female rats had similar [ET.sub.B] expression (655 [+ or -] 201 vs. 567 [+ or -] 39 fmol/mg protein, respectively), whereas male rats had significantly higher [ET.sub.A] expression (436 [+ or -] 162 vs. 47 [+ or -] 29 fmol/mg protein, respectively; P inner medullary collecting duct; endothelin doi: 10.1152/ajprenal.00400.2013

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Gale Document Number: GALE|A350791013