Effect of liver receptor homolog-1 on cell apoptosis and steroid hormone secretion on granulosa cells from Hu sheep.

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Date: Jan. 2022
Publisher: Springer
Document Type: Report; Brief article
Length: 312 words

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Keywords: Lrh-1; Steroid hormones; Hu sheep; Granulosa cells Abstract The apoptosis of granulosa cells can result in follicular atresia, a key process in follicle selection and development. The related molecular mechanisms were explored to study the effects of liver receptor homolog-1 (Lrh-1) on apoptosis, sodium-titanium system, and steroid synthesis of granulosa cells from Hu sheep in vitro. After constructing, designing, and synthesizing the Lrh-1 overexpression vector, liposomes were used to transfect Hu sheep granulosa cells. Thereafter, qRT-PCR and Western blot were used to detect the mechanism of Lrh-1 on the titanium system and steroid synthesis of Hu sheep granulosa cells. The overexpression efficiency was determined by fluorescence 48 h after liposome transfection into the Hu sheep granulosa cells in vitro culture. The transfection efficiency was higher, hence providing a basis for subsequent experiments. However, the protein level and transcriptional level of Lrh-1 significantly increased after transfection with Lrh-1 overexpression vector in Hu sheep granulosa cells. We further revealed that after Lrh-1 overexpression in Hu sheep granulosa cells, the expression of the pro-apoptotic gene Bax decreased significantly, while that of the anti-apoptotic gene Bcl-2 increased significantly, as well as the sodium peptide system. Moreover, the expression levels of natriuretic peptide precursor A, B, and C (NPPA, NPPB, NPPC) all showed an upward trend, while the expression levels of steroid synthesis-related genes (P450arom and P450scc) decreased. The above results showed that Lrh-1 overexpression influenced the apoptosis, sodium peptide system, and steroid synthesis. Author Affiliation: (1) Jiangsu Agri-Animal Husbandry Vocational College, 225300, Taizhou, Jiangsu, People's Republic of China (2) Yangzhou Maternal and Child Health Care Service Center, The Affiliated Hospital of Yangzhou University Medical College, 225009, Yangzhou, Jiangsu, People's Republic of China (3) Jiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, 210095, Nanjing, Jiangsu, People's Republic of China (d) wanglihong345@126.com Article History: Registration Date: 08/16/2021 Received Date: 02/17/2021 Accepted Date: 08/09/2021 Online Date: 01/06/2022 Byline:

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Gale Document Number: GALE|A691177519