Display of Recombinant Proteins on Bacterial Outer Membrane Vesicles by Using Protein Ligation

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From: Applied and Environmental Microbiology(Vol. 84, Issue 8)
Publisher: American Society for Microbiology
Document Type: Article
Length: 65 words

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Abstract :

The article explores the coupling of purified proteins to the hemoglobin protease (Hbp) carrier after its translocation across the outer membrane using the recently developed SpyTag/SpyCatcher protein ligation system. Subsequent addition of purified proteins fused to the SpyCatcher domain resulted in efficient covalent coupling to Hbp-SpyTag.Using in addition the orthogonal SnoopTag/SnoopCatcher system, multiple antigen modules could be coupled to Hbp in a sequential ligation strategy.

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Gale Document Number: GALE|A542247218