Prevalence and characterization of extended-spectrum [beta]-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from raw vegetables retailed in Southern Thailand.

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From: PeerJ(Vol. 9)
Publisher: PeerJ. Ltd.
Document Type: Article
Length: 6,883 words
Lexile Measure: 1470L

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Abstract :

Background The increasing prevalence of broad-spectrum ampicillin-resistant and third-generation cephalosporin-resistant Enterobacteriaceae, particularly Escherichia coli and Klebsiella pneumoniae, has become a global concern, with its clinical impacts on both human and veterinary medicine. This study examined the prevalence, antimicrobial susceptibility, and molecular genetic features of extended-spectrum [beta]-lactamase (ESBL)-producing E. coli and K. pneumoniae isolates from 10 types of raw vegetables. Methods In total, 305 samples were collected from 9 markets in Nakhon Si Thammarat, Thailand, in 2020. Results ESBL-producing E. coli and K. pneumoniae isolates were found in 14 of the 305 samples obtained from 7 out of 10 types of vegetables (4.6% of the total). Further, 14 ESBL-producing E. coli (n=5/14) and K. pneumoniae isolates (n=9/14) (1.6% and 3.0%, respectively) were highly sensitive to [beta]-lactam/carbapenem antibiotics (imipenem, 100%). ESBL-producing E. coli (n=4) and K. pneumoniae isolates (n=8) were also sensitive to non-[beta]-lactam aminoglycosides (amikacin, 80.00% and 88.89%, respectively). ESBL producers were most resistant to [beta]-lactam antibiotics, including ampicillin (85.71%) and the cephalosporins cefotaxime and ceftazidime (64.29%). The most frequently detected gene in ESBL-producing E. coli and K. pneumoniae was bla.sub.SHV . However, two ESBL-producing E. coli isolates also carried three other ESBL-encoding variants, bla.sub.TEM , bla.sub.CTX-M1 , bla.sub.GES and bla.sub.TEM , bla.sub.SHV , bla.sub.CTX-M9 , which may be due to their association with food chains and humans. Discussion Indeed, our results suggest that raw vegetables are an important source of ESBL-resistant E. coli and K. pneumoniae, which are potentially transmittable to humans via raw vegetable intake.

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Gale Document Number: GALE|A673122568