Uniform solid-phase extraction procedure for toxicological drug screening in serum and urine by HPLC with photodiode-array detection

Citation metadata

Date: Feb. 1997
From: Clinical Chemistry(Vol. 43, Issue 2)
Publisher: Oxford University Press
Document Type: Article
Length: 6,291 words
Lexile Measure: 1440L

Document controls

Main content

Abstract :

In this HPLC-diode-array detection method for toxicological drug screening, a mixed-mode solid-phase extraction procedure is optimized for isolation of a broad range of drugs from serum and urine. Basic, neutral, and weakly acidic drugs are uniformly recovered. The extract from the solid-phase cartridge is readily injected to a reversed-phase HPLC column for separation by gradient elution. Unknown drugs and metabolites in urine and serum samples from acute drug poisoning cases are rapidly identified by matching their retention times and ultraviolet spectra with hundreds of reference compounds in the library. Urine metabolites of common toxicants from various medications and drugs of abuse are recorded, with their changes of retention times and ultraviolet spectra as related to their metabolic transformations. Glucuronide conjugates of common benzodiazepines, tricyclic antidepressants, and beta-blockers are examined directly without chemical or enzymatic hydrolysis. The system is reliable for diverse clinical investigations of drug overdoses, drug-induced psychoses, and substance abuse. INDEXING TERMS: chromatography, reversed-phase * toxicology * abused drugs * drug metabolism and disposition

Source Citation

Source Citation   

Gale Document Number: GALE|A209621900