Abstract :
Keywords: Bilirubin glucuronidation; Dog; Human; Primary hepatocyte; Spheroid Abstract Species differences in bilirubin glucuronidation activity are observed between humans and dogs through liver microsomes and recombinant UDP-glucuronosyltransferase 1A1. Humans exhibit higher activity than that of dogs. In this study, bilirubin glucuronidation activity was examined in canine and human primary hepatocyte spheroids formed using a 3D culture system. When spheroid development in canine and human primary hepatocytes was evaluated on days 7 and 14 after the start of culture, canine primary hepatocyte spheroids had a more distinct spherical shape than human hepatocyte spheroids, irrespective of the culture period. Furthermore, mono- and di-glucuronide generation detected in spheroids were significantly higher (P Author Affiliation: (1) Bio-Innovation Research Center, Tokushima University, Tokushima, Japan (2) Preclinical Basic Research, Taiho Pharmaceutical Co., Ltd, Tokushima, Japan (3) Discovery and Preclinical Research Division, Taiho Pharmaceutical Co., Ltd, Tokushima, Japan (4) Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan (g) otoi@tokushima-u.ac.jp Article History: Registration Date: 07/15/2022 Received Date: 04/14/2022 Accepted Date: 07/13/2022 Online Date: 08/01/2022 Byline: