The effect of thermochemotherapy with mitomycin C on normal bladder urothelium, an experimental study

Citation metadata

From: International Urology and Nephrology(Vol. 48, Issue 1)
Publisher: Springer
Document Type: Report
Length: 350 words

Document controls

Main content

Abstract :

Byline: Murat Ucar (1), Muammer Altok (1), Mehmet Umul (1), Dilek Bayram (2), A[degrees]lkay Armagan (2), Mustafa GuneA (1), Tahsin Capkin (1), Sedat Soyupek (1) Keywords: Bladder; Chemotherapy; Hyperthermia; Mitomycin C; Thermochemotherapy Abstract: Purpose To investigate the effects of thermochemotherapy with mitomycin C (MMC) on normal rabbit bladder urothelium and to compare it with standard intravesical MMC and hyperthermia with normal saline. Methods Twenty-four male New Zealand rabbits, with a mean weight of 2.7 kg (in weight of 2.1--4.3 kg), were divided into three groups, each containing eight rabbits. Thermotherapy with only normal saline was performed in the first group, standard intravesical MMC was performed in the second group, and thermotherapy with MMC was performed in the last group. A week after the primary procedure, total cystectomy was performed and tissue samples were evaluated. Results The presence of epithelial vacuolar degeneration (p = 0.001), epithelial hyperplasia (p = 0.000), subepithelial fibrosis (p = 0.001) and hemorrhagic areas in the connective tissue (p = 0.002) was observed statistically significantly higher in the standard MMC group than in thermotherapy with normal saline group. There was almost a significant difference among standard MMC and normal saline group in terms of vascular congestion in the connective tissue (p = 0.08). Presence of epithelial vacuolar degeneration (p = 0.002), epithelial hyperplasia (p = 0.002), subepithelial fibrosis (p = 0.030), hemorrhagic areas (p = 0.011) and vascular congestion (p = 0.36) in the connective tissue was observed statistically significantly higher in the thermochemotherapy with MMC group than in standard intravesical MMC group. Polymorphonuclear cell infiltration was not considerable in any of the groups, and there was no significant difference between each groups (p = 0.140). Conclusion Administration of intravesical MMC causes a toxic effect on the normal urothelium of the bladder rather than an inflammatory reaction. Heating MMC significantly increased this effect. Author Affiliation: (1) Department of Urology, Faculty of Medicine, Suleyman Demirel University, 32260, Isparta, Turkey (2) Department of Histology and Embryology, Faculty of Medicine, Suleyman Demirel University, 32260, Isparta, Turkey Article History: Registration Date: 13/10/2015 Received Date: 09/09/2015 Accepted Date: 13/10/2015 Online Date: 26/10/2015

Source Citation

Source Citation   

Gale Document Number: GALE|A439283352