Effects of diazinon at different doses on rat liver and pancreas tissues

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From: Pesticide Biochemistry and Physiology(Vol. 87, Issue 2)
Publisher: Elsevier B.V.
Document Type: Author abstract
Length: 368 words
Lexile Measure: 60L

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Abstract :

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.pestbp.2006.06.011 Byline: Alpaslan Gokcimen (a), Kanat Gulle (a), Hilmi Demirin (b), Dilek Bayram (a), Ahmet Kocak (a), Irfan Altuntas (b) Keywords: Diazinon; Pancreas; Liver; Histopathological and biochemical changes Abstract: Diazinon is one of the most widely used organophosphates in agriculture. Toxic effects of diazinon are due to the inhibition of acetylcholinesterase, an enzyme needed for proper nervous system function. This study was designed to investigate the effects of diazinon at different doses on pancreas and liver tissues and in which dose level diazinon shows its effects. Sixty male Wistar albino rats were included in this study. Animals were initially divided into control and diazinon given groups. There were 10 animals in the control group and 50 animals in diazinon administered group. The latter was divided into five equal subgroups: 25, 50, 100, 200 and 300mg/kg of diazinon administered groups. Control group was given only saline. All animals in 300mg/kg diazinon group died. After 24h, rats were sacrificed under ether anesthesia. Tissue and blood samples were taken for biochemical and histopathological analysis. Sample tissues were examined under light microscope. In biochemical analysis, AST, ALT, LDH, amylase and lipase enzyme activities were measured. One-way ANOVA test was used to compare the groups. In 200mg/kg diazinon given group, it has been observed some histopathological changes in pancreas and liver tissues. Cholinesterase activities were significantly decreased and alkaline phosphatase levels were increased in all diazinon given groups, when compared with the controls. There was statistically significant difference between the control and diazinon given groups by means of serum amylase, lipase, ALT and AST activities (p Author Affiliation: (a) Department of Histology and Embryology, Suleyman Demirel University School of Medicine, Isparta, Turkey (b) Department of Biochemistry, Suleyman Demirel University School of Medicine, Isparta, Turkey Article History: Received 3 May 2006; Accepted 26 June 2006

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Gale Document Number: GALE|A160545776