Panax Notoginseng Saponin Attenuates Gastric Mucosal Epithelial Cell Injury Induced by Dual Antiplatelet Drugs through COX and PI3K/Akt/ VEGF-GSK-3[beta]-RhoA Network Pathway.

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From: Chinese Journal of Integrative Medicine(Vol. 27, Issue 11)
Publisher: Springer
Document Type: Report; Brief article
Length: 305 words

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Keywords: Panax notoginseng saponins; dual antiplatelet; permeability; cyclooxygenase; PI3K Abstract Objective To elucidate the underlying mechanism of Panax notoginseng saponin (PNS) on gastric epithelial cell injury and barrier dysfunction induced by dual antiplatelet (DA). Methods Human gastric mucosal epithelial cell (GES-1) was cultured and divided into 4 groups: a control, a DA, a PNS+DA and a LY294002+PNS+DA group. GES-1 apoptosis was detected by flow cytometry, cell permeability were detected using Transwell, level of prostaglandins E2 (PGE2), 6-keto-prostaglandin F1[alpha] (6-keto-PGF1[alpha]) and vascular endothelial growth factor (VEGF) in supernatant were measured by enzyme linked immunosorbent assay (ELISA), expression of phosphatidylinositide 3-kinase (PI3K), phosphorylated-PI3K (p-PI3K), Akt, phosphorylated-Akt (p-Akt), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), glycogen synthase kinase-3[beta] (GSK-3[beta]) and Ras homolog gene family member A (RhoA) were measured by Western-blot. Results DA induced apoptosis and hyper-permeability in GES-1, reduced supernatant level of PGE2, 6-keto-PGF1[alpha] and VEGF (P Conclusions PNS attenuates the suppression on COX/PG pathway from DA, alleviates DA-induced GES-1 apoptosis and barrier dysfunction through PI3K/Akt/ VEGF-GSK-3[beta]-RhoA network pathway. Author Affiliation: (1) Beijing First Hospital of Integrated Chinese and Western Medicine, 100039, Beijing, China (2) Center for Cardiovascular Disease, Xiyuan Hospital, China Academy of Chinese Medical Sciences, 100091, Beijing, China (3) Wangjing Hospital, China Academy of Chinese Medical Sciences, 100102, Beijing, China (f) billhappy@yeah.net Article History: Registration Date: 01/14/2021 Accepted Date: 12/18/2019 Online Date: 01/15/2021 Byline: Ming-ming Wang (1), Mei Xue (2), Zhong-hai Xin (3), Yan-hui Wang (1), Rui-jie Li (1), Hong-yan Jiang (corresponding author) (1, f), Da-zhuo Shi (2)

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Gale Document Number: GALE|A681341745