To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1016/j.pan.2016.03.001 Byline: Senay Topsakal (a), Ozlem Ozmen [email@example.com] (b,*), Fatma Nihan Cankara (c), Sukriye Yesilot (d), Dilek Bayram (e), Nilufer Genc Ozdamar (f), Sumeyra Kayan (f) Keywords High-fructose corn syrup; Oxidative stress; Pancreas; Pathology; Alpha lipoic acid; Immunohistochemistry Abstract Objectives Chronic consumption of high-fructose corn syrup (HFCS) causes several problems such as insulin resistance. The goal of the study was to investigate pancreatic damage induced by chronic HFCS consumption and the protective effects of alpha lipoic acid (ALA) on pancreatic cells. Methods Wistar Albino, 4-month-old, female rats weighing 250--300 g were randomly distributed into three groups, each containing eight rats. The study included an HFCS group, an HFCS + ALA-administered group and a control group (CON). The prepared 30% solution of HFCS (F30) (24% fructose, 28% dextrose) was added to the drinking water for 10 weeks. ALA treatment was begun 4 weeks after the first HFCS administration (100 mg/kg/oral, last 6 weeks). Rats were anaesthetised and euthanised by cervical dislocation 24 h after the last ALA administration. Blood samples for biochemical tests (amylase, lipase, malondialdehyde (MDA) and catalase (CAT)) and tissue samples for histopathological and immunohistochemical examinations (caspase-3, insulin and glucagon) were collected. Results Comparing the control and HFCS groups, serum glucose (150.92 [plus or minus] 39.77 and 236.50 [plus or minus] 18.28, respectively, p Conclusions This study indicated HFCS induced pancreatic lesions, but ALA had ameliorative effects. Author Affiliation: (a) Pamuklale University, Department of Endocrinology and Metabolism, Denizli, Turkey (b) Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Pathology, Burdur, Turkey (c) Suleyman Demirel University, Faculty of Medicine, Department of Pharmacology, Isparta, Turkey (d) Mehmet Akif Ersoy University, Bucak School of Health, Department of Nursing, Burdur, Turkey (e) Suleyman Demirel University, Faculty of Medicine, Department of Histology and Embryology, Isparta, Turkey (f) Suleyman Demirel University, Faculty of Medicine, Department of Physiology, Isparta, Turkey * Corresponding author. Mehmet Akif Ersoy University, Faculty of Veterinary Medicine, Department of Pathology, Istiklal Yerleskesi, 15030, Burdur, Turkey. Tel.: +90 2482132170.