Expression of Concern: Sox9 Potentiates BMP2-Induced Chondrogenic Differentiation and Inhibits BMP2-Induced Osteogenic Differentiation.

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Date: Apr. 1, 2021
From: PLoS ONE(Vol. 16, Issue 4)
Publisher: Public Library of Science
Document Type: Editorial
Length: 756 words
Lexile Measure: 1490L

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Author(s): The PLOS ONE Editors

Following the publication of this article [1], concerns were raised regarding panels presented in Figs 1, 2 and 5. Specifically,

In the left panel of Fig 1C, the left side of the bands do not appear to align correctly with the right sides of the bands.

The Day 5 anti-[beta]-actin panel of Fig 1Db appears similar to the Day 5 [beta]-actin panel of Fig 5B.

The Sox9 panel and the GFP panel of Fig 2C partially overlap.

The Day 2 [beta]-actin panel of Fig 5B appears similar to the Day 14 [beta]-actin panel of Fig 5C when flipped.

Fig 2. Sox9 potentiates BMP2-induced glycosaminoglycans synthesis in MSCs in micromass cultures. (A): Real-time PCR for the expression of chondrogenic differentiation marker gene ACAN were conducted on day 7 and day 14 after infection of AdGFP, AdBMP2, and/or AdSox9, using GAPDH as a house keeping gene. (B-C): Alcian blue staining for sulfated glycosaminoglycans in micromass cultures of C3H10T1/2 cells on day 7 and day 14 after transduction of indicated recombinant adenoviruses, gross observation (B) and microscope examination (C, 40X) are shown. (D): Alcian blue staining quantifying:...

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Gale Document Number: GALE|A657069343