Abstract :
A three-layered microfluidic chip is fabricated and evaluated for studying the dynamics of neurotransmitter release from PC-12 cells. Monitoring neurotransmitter release from PC-12 cells exposed to KCl (or alcohol) reveals that all four neurotransmitters investigated are released. These results suggest that DA and 5-HT are packaged into different vesicle pools and they are mobilized differently in response to chemical stimuli.