Study on anti-inflammatory efficacy and correlative ingredients with pharmacodynamics detected in acute inflammation rat model serum from Caulis Lonicerae japonicae

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Date: June 1, 2016
Publisher: Urban & Fischer Verlag
Document Type: Report
Length: 7,457 words
Lexile Measure: 1630L

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ABSTRACT

Background: Caulis Lonicerae japonicae (CLJ) is often used for the treatment of inflammation such as acute fever, headache, respiratory infection and epidemic diseases. Nevertheless, domestic and foreign researches simply fail to focus on reports of CLJ, especially its anti-inflammatory effects and correlative components.

Purpose: In this study, we investigated anti-inflammatory effects and serum components of Caulis Lonicerae japonicae (CLJ) in models of acute inflammation and correlative analysis between anti-inflammatory effects and serum components of CLJ in rat serum to analyze the changes in the relative contents of components in serum with time and in the corresponding values to characterize active fractions of CLJ and identify the major active components of CLJ in rats.

Study design: Active fractions of CLJ were screened using xylene-induced ear oedema mice model. Anti-inflammatory effects were evaluated using carrageenan-induced paw oedema rat model, and then correlative components in rat serum were demonstrated with HPLC-QTOF/MS/MS method explored.

Methods: HPLC-QTOF/MS/MS was developed to analyze the components absorbed in rat serum after oral administration of CLJ.

Results: Ethyl acetate extracts (ECLJ) and n-butanol extracts (BCLJ) of CLJ were preliminarily screened as active fractions of CLJ (EBCLJ) using xylene-induced ear oedema mice model, and effectively inhibited edema and values of interleukin-1 (IL-1), nuclear factor-kappaB (NF-[kappa]B), tumor necrosis factor-[alpha] (TNF- [alpha]) activities and prostaglandinE2 (PG[E.sub.2]) productions using carrageenan-induced paw oedema rat model. 33 peaks were found in total ion current chromatograms of EBCLJ, and 27 components were observed in EBCLJ-treated rat serum, only 11 of which were correlated with anti-inflammatory effects.

Conclusions: The present study contributes to the study on the pharmacodynamic basis of CLJ and provides potent evidence for developing CLJ as a safe and promising natural drug in inflammation treatment.

Keywords:

Caulis Lonicerae japonicae

Active fractions

Anti-inflammatory effect

Serum components

Correlation analysis between

anti-inflammatory effects and serum components

Introduction

Lonicera japonica Thunb. (LJT) is a commonly used medicinal plant that plays an important role in the clinical treatment of Traditional Chinese medicine (TCM). Caulis Lonicerae japonicae (CLJ), Rendongteng which is derived from the dried caulis of LJT, has long been used for the treatment of acute fever, headache, respiratory infection and epidemic diseases (Chinese Pharmacopoeia Committee, 2010). Meanwhile, CLJ for external use could clinically cure hemorrhoids, anal oedema and prostatitis, while CLJ juice for external use could treat dose-fractured limbs, eliminate oedema and promote bone recovery significantly (Lu, 2012).

Flos Lonicerae japonicae (FLJ), Jinyinhua which is derived from the dried flower buds of LJT, has also been used for the treatment of exopathogenic wind-heat or epidemic febrile diseases, sores and furuncles for centuries. Also they are specially considered two totally different medicines in Chinese pharmacopoeia after 2000. Phytochemical investigations on CLJ and FLJ, different parts of the same plant, have discovered that phenolic acids and iridoid glycosides were the common components in both parts, like chlorogenic acid, loganin, sweroside, etc., while their relative contents revealed large differences (Zhang et al., 2015) and flavonoids were hardly detected in CLJ (Ye et al., 2014). Although they are partly similar in...

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Gale Document Number: GALE|A456907642