24-Epibrassinolide and methyl jasmonate can encourage cell growth and the production of secondary metabolites in immobilized cells of Hyoscyamus niger.

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Date: Oct. 2022
Publisher: Springer
Document Type: Report; Brief article
Length: 240 words

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Keywords: Hyoscyamus niger; Immobilized cell; 24-epibrassinolide; Methyl jasmonate; Tropane alkaloids; Phenolics Abstract This study was carried out to determine the effects of 24-epibrassinolide (24-eBL) and methyl jasmonate (MeJA) on cell growth and accumulation of secondary metabolites in immobilized cells of Hyoscyamus niger. For this purpose, different combinations of 24-eBL (0.5, 1.0, and 2.0 mg l.sup.-1) and MeJA (1.0 mM) were applied to cells immobilized with calcium alginate. At the end of the 30-day applications, fresh and dry cell weights, cell growth index, tropane alkaloids (hyoscyamine and scopolamine), phenolic (gallic acid, catechin, chlorogenic acid, caffeic acid, epicatechin, vanillin, p-coumaric acid, o-coumaric acid, ferulic acid, rutin, cinnamic acid, rosmarinic acid, and quercetin), and total phenolic content were determined. According to the results, 2.0 mg l.sup.-1 24-eBL for cell growth, 0.5 mg l.sup.-1 24-eBL for scopolamine and hyoscyamine, and all 24-eBL combinations with 1.0 mM MeJA for phenolic compounds were selected as the most effective applications. In conclusion, it was determined that the application of 24-eBL and MeJA may be successfully used to increase the production of secondary metabolites in immobilized cell cultures of H. niger when used in appropriate concentrations and combinations. Graphical abstract Author Affiliation: (1) Department of Agricultural Biotechnology, Faculty of Agriculture, Isparta University of Applied Sciences, 32270, Isparta, Turkey (2) Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Suleyman Demirel University, Isparta, Turkey (b) tunhandemirci@sdu.edu.tr Article History: Registration Date: 10/12/2022 Received Date: 08/31/2022 Accepted Date: 10/12/2022 Online Date: 11/14/2022 Byline:

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Gale Document Number: GALE|A727538989