Inokosterone Is A Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients: Analysis from Active Ingredient of Cyathula Officinalis.

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From: Chinese Journal of Integrative Medicine(Vol. 27, Issue 10)
Publisher: Springer
Document Type: Report; Brief article
Length: 285 words

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Keywords: rheumatoid arthritis; estrogen receptor 1; inokosterone; Cyathula Officinalis; Chinese medicine Abstract Objective To elucidate the active compounds and the molecular mechanism of Cyathula Officinalis as a drug treatment for rheumatoid arthritis (RA). Methods The target genes of active ingredients from Cyathula Officinalis were obtained from bioinformatics analysis tool for the molecular mechanism of traditional Chinese medicine. The protein-protein interaction between the target genes were analyzed using STRING and Genemania. The transcriptome of RA patients compared to healthy people (GSE121894) were analyzed using R program package Limma. The relative expression of the target genes was obtained from the RNA-seq datasets. The molecular docking analyses were processed based on the molecular model of estrogen receptor 1 (ESR1) binding with estradiol (PDB ID:1A52). The binding details were analyzed by SYBYL. Results Inokosterone, ecdysterone, and cyaterone were the 3 active ingredients from Cyathula Officinalis that bind to target genes. Of all the significantly changed genes from RA patients, ESR1, ADORA1, and ANXA1 were significantly increased in mRNA samples of RA patients. Conclusion ESR1, the transcription factor that binds inokosterone in the molecular binding analysis, is the target protein of Cyathula Officinalis. Author Affiliation: (1) Department of Medical Laboratory, Luoyang Orthopedic Hospital of Henan Province, Orthopedic Institute of Henan Province, 471002, Luoyang, Henan Province, China (2) Miller School of Medicine, University of Miami, 33136, Miami, FL, USA (3) Institute of Technical Biology & Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, 230031, Hefei, China (4) Eatobe Pte. Limited, 573969, Singapore, Singapore (f) lxw561@miami.edu Article History: Registration Date: 08/24/2021 Accepted Date: 05/11/2020 Online Date: 08/25/2021 Byline: Ji-hao Mo (1), Han-kun Xie (2), Ye-mian Zhou (3), Sihan-benjamin Ng (4), Shao-xia Li (1), Lei Wang (corresponding author) (2, f)

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Gale Document Number: GALE|A681422108