Extract from Zanthoxylum piperitum Induces Apoptosis of AGS Gastric Cancer Cells Through Akt/MDM2/p53 Signaling Pathway.

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From: Chinese Journal of Integrative Medicine(Vol. 27, Issue 10)
Publisher: Springer
Document Type: Report
Length: 411 words

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Keywords: AGS gastric cancer cells; Zanthoxylum piperitum; apoptosis; Akt/MDM2/p53 signaling pathway; active compounds Abstract Objective To determine the effect of Zanthoxylum piperitum extracet (ZPE) on apoptosis and analyze anticancer substances in ZPE, changes in proteins related to apoptosis, and pathological changes in tumors in mouse. Methods Fifteen 4-week-old female BALB/c nu/nu mice were divided into 3 groups depending on ZPE dose, with 5 in each group. AGS gastric carcinoma cells (1 × 10.sup.6 cells/200 µL) were subcutaneously injected into the flank of each mouse. One week after the injection of AGS cells, ZPE was administered to the skin tissue [10 or 50 mg/(kg*d)] in the low- and high-dose groups, respectively for 20 days. Control animals were injected with vehicle only. After 3 weeks, the tumor was extracted and carried out for immunohistochemistry, the tendency of apoptosis and p53 in the body was checked using TdT-mediated dUTP nick-end labeling (TUNEL) assay. For 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, annexin V dead cell staining, cell cycle arrest and Western blotting, AGS gastric carcinoma cells were incubated with various concentrations of ZPE for 24 h. Cell survival rates were analyzed by MTT assays. Apoptosis was analyzed using annexin V dead cell staining and cell cycle arrest and measured using Muse cell analyzer. Results High performance liquid chromatography (HPLC) analysis showed that ZPE contained organic sulfur compounds such as alliin and S-allylcysteine. MTT assay results revealed that ZPE (10--85 µ g/mL) could effectively inhibit the growth of AGS gastric cancer cells at higher concentrations (P Conclusion ZPE decreases AGS cell proliferation and induces apoptosis by inhibiting Akt and MDM2 expression. Author Affiliation: (1) Department of Biological science and Biotechnology, College of Life science and Nano Technology, Hannam University, 1646 Yuseong-daero, Yuseong-gu, 34054, Daejeon, South Korea (2) Department of Food Science & Bio Technology, Shinansan University, Deahakro Danwon-gu, Ansan-City, Gyenggi-do, Korea (f) ym1938@naver.com Article History: Registration Date: 07/27/2021 Accepted Date: 09/23/2019 Online Date: 07/28/2021 Byline: Ye Seul Park (1), Gun He Nam (1), Kyung Jo Jo (1), Hye Won Kawk (1), Sang Yung Kim (2), Young Min Kim (corresponding author) (1, f)

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Gale Document Number: GALE|A681422105