Protective Effect of Naoxintong Capsule (èå¿éè¶å) Combined with Guhong Injection (谷红注å°æ¶²) on Rat Brain Microvascular Endothelial Cells during Cerebral Ischemia-Reperfusion Injury.

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Date: Oct. 2021
From: Chinese Journal of Integrative Medicine(Vol. 27, Issue 10)
Publisher: Springer
Document Type: Report; Brief article
Length: 380 words

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Keywords: cerebral ischemia/reperfusion injury; Naoxintong Capsule; Guhong Injection; brain microvascular endothelial cells; apoptosis; rat Abstract Objective To investigate the synergistic effect of Naoxintong Capsule (NXTC, èå¿éè¶å) and Guhong Injection (GHI, 谷红注å°æ¶²) on cerebral ischemia-reperfusion (I/R) injury. Methods Forty-eight Sprague-Dawley rats were divided into 6 groups: control group, oxygen and glucose deprivation (OGD) group, nimodipine group (9.375 mg/kg), NXTC group (0.5 g/kg), GHI group (5 mL/kg) and NXTC+GHI group (0.5 g/kg NXTC+5 mL/kg GHI), after the onset of reperfusion and once per day for the following 7 days. Blood was collected 1 h after final administration, and the sera were collected. Cultured primary rat brain microvascular endothelial cells (rBMECs) were subjected to OGD to establish a cell injury model. Untreated rBMECs were used as blank control. The cell counting kit-8 assay was used to assess cell viability using the sera. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were assessed using an enzyme-linked immunosorbent assay. Apoptosis was evaluated after Hoechst33342 staining using fluorescence microscopy and flow cytometry. JC-1 staining was performed to assess changes in mitochondrial membrane potential. Results Statistical analysis indicated that more than 95% of the cells were rBMECs. Compared with the OGD group, the cellular morphology of the all drug delivery groups improved. In particular, the combined drug group had the most significant effect. Compared with the OGD group, all drug intervention groups induced a decrease in the apoptotic rate of rBMECs, increased the SOD levels, and decreased the MDA levels (all P Conclusion The combinationa application of NXTC and GHI on cerebral I/R injury clearly resulted in protective benefits. Author Affiliation: (1) College of Life Science, Zhejiang Chinese Medical University, 310053, Hangzhou, China (2) College of Pharmacy, Zhejiang Chinese Medical University, 310053, Hangzhou, China (3) College of Basic Medicine, Zhejiang Chinese Medical University, 310053, Hangzhou, China (g) Article History: Registration Date: 04/03/2020 Accepted Date: 08/31/2019 Online Date: 04/04/2020 Byline: Hai-yan Wang (1), Hui-fen Zhou (1), Yu He (2), Li Yu (1), Chang Li (1), Jie-hong Yang (3), Hai-tong Wan (corresponding author) (1, g)

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Gale Document Number: GALE|A681422102