Inhibition of peroxisome proliferator-activated receptor gamma prevents the melanogenesis in murine B16/F10 melanoma cells

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Publisher: Hindawi Limited
Document Type: Report
Length: 5,948 words
Lexile Measure: 1250L

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Abstract :

The purpose of this study was to investigate if PPAR[gamma] plays a role in the melanogenesis. B16/F10 cells were divided into five groups: control, melanin stimulating hormone ([alpha]-MSH), [alpha]-MSH+retinol, [alpha]-MSH+GW9662 (PPAR[gamma] antagonist), and GW9662. Cells in the control group were cultured in the Dulbecco's modified Eagle's medium (DMEM) for 48hrs. To initiate the melanogenesis, cells in all [alpha]-MSH groups were cultured in medium containing [alpha]-MSH (10 nM) for 48 hrs. Cells were treated simultaneously with retinol (5 [micro]M) in the [alpha]-MSH+retinol group. Instead of retinol, GW9662 (10 [micro]M) was cocultured in the [alpha]-MSH+GW9662 group. Cells in the final group were cultured in the DMEM with GW9662. All the analyses were carried out 48 hours after treatments. The [alpha]-MSH was able to increase cell number, melanin production, and the activity of tyrosinase, the limiting enzyme in melanogenesis. These [alpha]-MSH-induced changes were prevented either by retinol or by GW9662. Further analyses of the activities of antioxidant enzymes including glutathione, catalase, and the superoxide dismutase (SOD) showed that [alpha]-MSH treatment raised the activity of SOD which was dependent on PPAR[gamma] level. According to our results, the [alpha]-MSH-induced melanogenesis was PPAR[gamma] dependent, which also modulated the expression of SOD.

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Gale Document Number: GALE|A427024101