Cyanobacteria in hot pursuit: Characterization of cyanobacteria strains, including novel taxa, isolated from geothermal habitats from different ecoregions of the world.

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Publisher: Elsevier B.V.
Document Type: Report
Length: 447 words

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Keywords Amphirytos necridicus gen. et sp. nov.; Hillbrichtia pamiria gen. et sp. nov.; Thermoleptolyngbya hindakiae sp. nov.; Thermotolerance; Nonribosomal Peptide Synthetases; Polyketide Synthases Highlights * 13 Cyanobacteria from thermal habitats were characterized by a polyphasic approach. * Taxa with sub-cosmopolitan expansion (Calothrix, Desertifilum and Trichormus). * Two new genera and three new species inhabiting geothermal environments. * Correlation between thermotolerance ability and genetic background. Abstract Well-studied thermal spring microbial mat systems continue to serve as excellent models from which to make discoveries of general importance to microbial community ecology in order to address comprehensively the question of "who is there" in a microbial community. Cyanobacteria are highly adaptable and an integral part of many ecosystems including thermal springs. In this context, we sampled disparate thermal springs, spanning from Iceland and Poland to Greece and Tajikistan. Thirteen (13) strains were isolated and characterised with taxonomic indices and molecular markers (16S-23S rRNA region and cpcBA gene), whilst their thermotolerance was evaluated. Screening for the presence of genes encoding three heat shock proteins, as well as non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) was performed. This approach resulted in the description of two new genera (Hillbrichtia and Amphirytos) and their type species (Hillbrichtia pamiria and Amphirytos necridicus) representing Oscillatoriales and Synechococcales orders, respectively. We also found unique lineages inside the genus Thermoleptolyngbya, describing a novel species (T. hindakiae). We described the presence of sub-cosmopolitan taxa (such as Calothrix, Desertifilum, and Trichormus). Strains were diverse concerning their thermophilic ability with the strains well adapted to high temperatures possessing all three investigated genes encoding heat shock proteins as well as studied PKS and NRPS genes. In this work, we show novel cyanobacteria diversity from thermal springs from disparate environments, possible correlation of thermotolerance and their genetic background, which may have implications on strategic focusing of screening programs on underexploited taxa in these habitats. Author Affiliation: (a) Institute of Environmental Biology, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02-089 Warsaw, Poland (b) Department of Botany, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece (c) Department of Hydrobiology, Institute of Environmental Biology, Faculty of Biology, Adam Mickiewicz University in Poznan, Uniwersytetu Poznanskiego 6, 61-614 Poznan, Poland (d) Institute of Botany, Plant Physiology and Genetics, Academy Science Republic of Tajikistan, 27 Karamov Str., Dushanbe 734017, Tajikistan * Corresponding author. Article History: Received 26 November 2021; Revised 18 January 2022; Accepted 4 March 2022 (footnote)1 The authors have equally contributed to this work. Byline: Iwona Jasser (a,1), Manthos Panou (b,1), Nataliia Khomutovska (a), Malgorzata Sandzewicz (a), Emmanuel Panteris (b), Toirbek Niyatbekov (d), Lukasz Lach (a), Jan Kwiatowski (a), Mikolaj Kokocinski (c), Spyros Gkelis [sgkelis@bio.auth.gr] (b,*)

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Gale Document Number: GALE|A699113568