Dengue-2-virus-interacting polypeptides involved in mosquito cell infection

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From: Archives of Virology(Vol. 155, Issue 9)
Publisher: Springer
Document Type: Report
Length: 197 words

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Byline: Mandar S. Paingankar (1), Mangesh D. Gokhale (2), Dileep N. Deobagkar (1,3) Abstract: For the design of effective antiviral strategies, understanding the fundamental steps of the virus life cycle, including virus--host interactions, is essential. We performed a virus overlay protein binding assay followed by proteomics for identification of proteins from membrane fractions of A7 (Aedes aegypti) cells, C6/36 (Aedes albopictus) cells and the midgut brush border membrane fraction of Ae. aegypti mosquito that bind to dengue-2 virus. Actin, ATP synthase [beta] subunit, HSc 70, orisis, prohibitin, tubulin [beta] chain, and vav-1 were identified as dengue-2-virus-binding proteins. Our results suggest that dengue-2 virus exploits an array of housekeeping proteins for its entry in mosquito cells. Author Affiliation: (1) Molecular Biology Research Laboratory, Department of Zoology, Centre for Advance Studies, University of Pune, Pune, 411007, India (2) Department of Medical Entomology and Zoology, National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune, 411001, India (3) Goa University, Taleigaon Plateau, Goa, 403206, India Article History: Registration Date: 12/06/2010 Received Date: 23/04/2009 Accepted Date: 12/06/2010 Online Date: 23/06/2010 Article note: Electronic supplementary material The online version of this article (doi: 10.1007/s00705-010-0728-7) contains supplementary material, which is available to authorized users.

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Gale Document Number: GALE|A237606205