Transcriptomic data reveals nuclear-mitochondrial discordance in Gomphocerinae grasshoppers (Insecta: Orthoptera: Acrididae).

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Publisher: Elsevier B.V.
Document Type: Report; Brief article
Length: 389 words

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Keywords Caelifera; Chorthippus; Coalescent; Molecular clock; Pseudochorthippus; Taxonomy Highlights * Previous phylogenies of Gomphocerinae grasshoppers based on few markers were poorly resolved. * New sequencing and analytical methods allow incorporating gene tree discordance. * Molecular clock confirms a young radiation around 1.38 mya. * Unlike mitogenomes, a nuclear species tree retrieves species as monophyletic. * Transcriptomics resolve phylogenetic relationships despite incomplete lineage sorting. Abstract The phylogeny of many groups of Orthoptera remains poorly understood. Previous phylogenetic studies largely restricted to few mitochondrial markers found many species in the grasshopper subfamily Gomphocerinae to be para- or polyphyletic, presumably because of incomplete lineage sorting and ongoing hybridization between putatively young lineages. Resolving the phylogeny of the Chorthippus biguttulus species complex is important because many morphologically cryptic species occupy overlapping ranges across Eurasia and serve important ecological functions. We investigated whether multispecies coalescent analysis of 540 genes generated by transcriptome sequencing could resolve the phylogeny of the C. biguttulus complex and related Gomphocerinae species. Our divergence time estimates confirm that Gomphocerinae is a very young radiation, with an age estimated at 1.38 (2.35--0.77) mya for the C. biguttulus complex. Our estimated topology based on complete mitogenomes recovered some species as para- or polyphyletic. In contrast, the multispecies coalescent based on nuclear genes retrieved all species as monophyletic clusters, corroborating most taxonomic hypotheses. Our results underline the importance of using nuclear multispecies coalescent methods for studying young radiations and highlight the need of further taxonomic revision in Gomphocerinae grasshoppers. Author Affiliation: (a) Leibniz Institute for the Analysis of Biodiversity Change (LIB), Zoological Museum Hamburg, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany (b) Zoologische Staatssammlung (SNSB-ZSM), Münchhausenstr. 21, 81247 Munich, Germany (c) Department Ecology & Ecosystem Management, Plant Biodiversity Research, Technical University of Munich, Emil-Ramann Strasse 2, 85354 Freising, Germany (d) Museum of Southwestern Biology and Department of Biology, University of New Mexico, MSC03-2020, 1 University of New Mexico, Albuquerque, NM, USA (e) Division of Evolutionary Biology, Faculty of Biology II, Ludwig-Maximilians-Universität München, Grosshaderner Strasse 2, 82152 Planegg-Martinsried, Germany * Corresponding author at: Leibniz Institute for the Analysis of Biodiversity Change (LIB), Zoological Museum, Martin-Luther-King-Platz 3, 20146 Hamburg, Germany. Article History: Received 8 March 2021; Revised 2 February 2022; Accepted 3 February 2022 Byline: Oliver Hawlitschek [oliver.hawlitschek@gmx.de] (a,b,*), Edgardo M. Ortiz (c), Sajad Noori (a), Kathleen C. Webster (b,d), Martin Husemann (a), Ricardo J. Pereira (e)

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Gale Document Number: GALE|A699113561