Rates of fluoroquinolone resistance in domestically acquired Campylobacter jejuni are increasing in people living within a model study location in Canada

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Date: Jan. 2021
From: Canadian Journal of Microbiology(Vol. 67, Issue 1)
Publisher: NRC Research Press
Document Type: Report
Length: 11,321 words
Lexile Measure: 1480L

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Abstract :

Antimicrobial resistance was evaluated in Campylobacter jejuni isolated from 1291 diarrheic people over a 15-year period (2004-2018) in southwestern Alberta, a model location in Canada with a high rate of campylobacteriosis. The prevalence of resistance to chloramphenicol, clindamycin, erythromycin, and gentamicin was low during the examination period ([less than or equal to] 4.8%). Resistance to tetracycline remained consistently high (41.6%-65.1%), and resistance was primarily conferred by plasmid-borne tetO (96.2%). Resistance rates to ciprofloxacin and nalidixic acid increased substantially over the examination period, with a maximal fluoroquinolone resistance (FQR) prevalence of 28.9% in 2016. The majority of C. jejuni isolates resistant to ciprofloxacin (93.9%) contained a C257T single nucleotide polymorphism within the gyrA chromosomal gene. Follow up with infected people indicated that the observed increase in FQR was primarily due to domestically acquired infections. Moreover, the majority of FQ-resistant C. jejuni subtypes (82.6%) were endemic in Canada, primarily linked to cattle and chicken reservoirs; 18.4% of FQ-resistant isolates were assigned to three subtypes, predominantly associated with cattle. Study findings indicate the need to prioritize FQR monitoring in C. jejuni infections in Canada and to elucidate the dynamics of the emergence and transmission of resistant C. jejuni strains within and from cattle and chicken reservoirs. Keywords: antimicrobial resistance, human health risk, zoonotic reservoirs, cattle, One Health approach. La resistance aux antimicrobiens a ete evaluee chez Campylobacter jejuni isole de 1291 personnes diarrheiques sur une periode de 15 ans (2004-2018) dans le sud-ouest de l'Alberta, un endroit type au Canada avec un taux eleve de campylobacteriose. La prevalence de la resistance au chloramphenicol, a la clindamycine, a l'erythromycine et a la gentamicine etait faible pendant la periode d'examen ([less than or equal to] 4,8%). La resistance a la tetracycline est restee constamment elevee (41,6-65,1%) et principalement conferee par tetO plasmidique (96,2%). Les taux de resistance a la ciprofloxacine et a l'acide nalidixique ont considerablement augmente au cours de la periode d'examen, avec une prevalence de resistance maximale aux fluoroquinolones de 28,9% en 2016. La majorite des isolats de C. jejuni resistants a la ciprofloxacine (93,9%) contenaient un polymorphisme mononucleotidique C257T dans le gene chromosomique gyrA. Le suivi des personnes infectees a indique que l'augmentation observee de la resistance aux fluoroquinolones etait principalement due a des infections acquises interieurement. En outre, la majorite des sous-types de C. jejuni (82,6%) resistants aux fluoroquinolones etaient endemiques au Canada, principalement lies aux reservoirs des bovins et des poulets; 18,4% des isolats resistants aux fluoroquinolones ont ete attribues a trois sous-types, principalement associes aux bovins. Les resultats de l'etude indiquent la necessite de donner la priorite a la surveillance de la resistance aux fluoroquinolones lors d'infections a C. jejuni au Canada, et d'elucider la dynamique de l'emergence et de la transmission des souches resistantes de C. jejuni dans et a partir des reservoirs de bovins et de poulets. [Traduit par la Redaction] Mots-cles : resistance antimicrobienne, risque pour la sante humaine, reservoirs de zoonoses, bovins, approche .

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Gale Document Number: GALE|A648001924