Abstract :
Keywords: Transferrin; ACOT7; Epidermal stem cells; Lipid peroxidation Abstract Epidermal stem cells (ESCs) are critical for skin regeneration and repair. Previous studies have shown that ESCs are susceptible to oxidative stress, which in turn leads to lipid peroxidation and affects skin repair. Our study aims to explore how ESCs resist lipid peroxidation. By performing proteomics analysis, we found that the expression of Acyl-CoA thioesterase 7 (ACOT7) was positively correlated with the concentration of transferrin. Overexpression adenovirus vectors of ACOT7 were constructed and transfected into ESCs. Levels of lipid peroxidation by flow cytometry, cell viabilities, and MDA levels were measured. The results revealed that ACOT7 could inhibit lipid peroxidation, reduce the level of malondialdehyde (MDA), and improve the survival rate of ESCs induced by [H.sub.2]O.sub.2, Erastin, and RSL3. Our data suggest that ACOT7 has an effect on protecting ESCs against iron-dependent lipid peroxidation. Author Affiliation: (1) Department of Plastic Surgery, Shandong Provincial Qianfoshan Hospital, Cheeloo College of Medicine, Shandong University, 250012, Jinan, Shandong, People's Republic of China (2) Jinan Clinical Research Center for Tissue Engineering Skin Regeneration and Wound Repair, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, 250014, Jinan, People's Republic of China (3) Department of Plastic Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, 250014, Jinan, People's Republic of China (j) ybwang@sdfmu.edu.cn Article History: Registration Date: 06/22/2022 Received Date: 01/31/2022 Accepted Date: 06/10/2022 Online Date: 08/29/2022 Byline: