Antimycobacterial screening of traditional medicinal plants using the microplate resazurin assay

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From: Canadian Journal of Microbiology(Vol. 56, Issue 6)
Publisher: NRC Research Press
Document Type: Report
Length: 4,364 words

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Abstract :

Multidrug-resistant Mycobacterium tuberculosis strains have rapidly become a global health concern. North American First Nations communities have used traditional medicines for generations to treat many pulmonary infections. In this study, we evaluated the antimycobacterial activity of 5 medicinal plants traditionally used as general therapeutics for pulmonary illnesses and specifically as treatments for tuberculosis. Aqueous extracts of Aralia nudicaulis, Symplocarpus foetidus, Heracleum maximum, Juniperus communis, and Acorus calamus were screened for antimycobacterial activity against Bacillus Calmette-Gueerin, Mycobacterium avium, and M. tuberculosis H37Ra using the colorimetric microplate resazurin assay. Extracts of Acorus calamus and H. maximum root demonstrated significant antimycobacterial activity comparable to that of the rifampin control (2 [micro]g/mL). Evaluation of the cytotoxicity of these 2 extracts using the MTT assay also showed that the extracts were less toxic to 3 human cell lines than was the DMSO positive control. This study demonstrates that aqueous extracts of the roots of H. maximum and Acorus calamus possess strong in vitro antimycobacterial activity, validates traditional knowledge, and provides potential for the development of urgently needed novel antituberculous therapeutics. Key words: tuberculosis, mycobacteria, Heracleum maximum, Acorus calamus. Les souches de Mycobacterium tuberculosis multiresistantes aux antibiotiques sont rapidement devenues un enjeu de sante mondiale. Les communautes des Premieres Nations d'Amerique du Nord ont utilise; des medicaments traditionnels depuis plusieurs generations pour traiter divers types d'infection pulmonaire. Dans cette etude, nous avons evaluee l'activite anti-mycobacterienne de 5 plantes medicinales utilisees traditionnellement comme medicaments gereraux contre les maladies pulmonaires, et specifiquement comme traitement de la tuberculose. Des extraits aqueux de Aralia nudicaulis, Symplocarpus foetidus, Heracleum maximum, Juniperus communis et Acorus calamus ont ete testes relativement a leur activite antimycobacterienne envers le Bacillus Calmette-Gueirin, Mycobacterium avium et M. tuberculosis H37Ra, par un dosage colorimetrique a la resazurine sur microplaque. Les extraits de racines de Acorus calamus et de H. maximum ont demontre une activite; anti-mycobacterienne significative, comparable a celle de la rifampicine (3 [micro]m/mL) utilisee comme controle. L'evaluation de la cytotoxicite de ces 2 extraits a l'aide d'un dosage au MTT a aussi montree qu'ils eetaient moins toxiques envers 3 ligneees cellulaires humaines que le DMSO utilise comme controle positif. Cette etude demontre que les extraits aqueux de racines de H. maximum et de Acorus calamus possedent une forte activite anti-mycobacterienne, confirmant les savoirs traditionnels, et elle ouvre la voie au developpement de nouveaux agents anti-tuberculeux, imperieusement necessaires. Mots-cles: tuberculose, mycobacteirie, Heracleum maximum, Acorus calamus. [Traduit par la Redaction]

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Gale Document Number: GALE|A230774476