Effects of hepatocyte growth factor (HGF) and activin a on the morphogenesis of rat submandibular gland-derived epithelial cells in serum-free collagen gel culture

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Publisher: Springer
Document Type: Article
Length: 244 words

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Byline: Miho Furue (1), Tetsuji Okamoto (2), Hiroyuki Hayashi (3), J. Denry Sato (4), Makoto Asashima (5), Shigeru Saito (1) Keywords: salivary gland; HGF; activin; branching morphogenesis Abstract: To study the mechanisms of morphogenesis in salivary gland regeneration, we have established the RSMG-1 cell line derived from submandibular gland (SMG) of 10-wk-old Wistar female rats in serum-free culture. Our finding that RSMG-1 cells originated from duct cells was based on morphology and immunohistochemical results. In three-dimensional serum-free collagen gel culture, HGF induced branching morphogenesis of RSMG-1 cells. Histological examination revealed that HGF-induced branching structure exhibited well-formed lumina. This morphology closely resembles that found in vivo. The cells also expressed activin A. Exogenously added activin A at a high concentration reduced HGF-induced branching morphogenesis. These findings suggest that the morphogenesis of the salivary gland is modulated by HGF and activin A. Our results show that the RSMG-1 cell line may be useful in studies of salivary gland regeneration. Author Affiliation: (1) Department of Oral Biochemistry, Kanagawa Dental College, 82 Inaoka-cho, 238-8580, Yokosuka City, Kanagawa, Japan (2) Department of Oral and Maxillofacial Surgery I, Hiroshima University School of Dentistry, 1-2-3 Kasumi, 734-0037, Hiroshima, Japan (3) Department of Oral Histochemistry, Kanagawa Dental College, USA (4) Adirondack Biomedical Res. Inst., Lake Placid, 12946, New York (5) Department of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, 153-0041, Tokyo, Japan Article History: Registration Date: 18/05/1999 Received Date: 06/08/1998 Accepted Date: 02/09/1998

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Gale Document Number: GALE|A206213016