Effects of serum containing Chinese medicine Sanpi Pingwei (aePSca13e) formula on proliferation and apoptosis of human SGC-7901 cells

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Date: Feb. 2013
From: Chinese Journal of Integrative Medicine(Vol. 19, Issue 2)
Publisher: Springer
Document Type: Article
Length: 344 words

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Abstract :

Byline: Xiao-yan Dang (1,2), Lei Dong (1), Hai-tao Shi (1), Bai-cang Zou (1) Keywords: stomach neoplasms; Chinese medicine; apoptosis Abstract: Objective To investigate the effects of serum containing Chinese medicine (CM) Sanpi Pingwei (aePSca13e, SPPW) formula on the proliferation and apoptosis of human SGC-7901 cells and the possible mechanism. Methods Serum containing CM SPPW formula (SPPW serum) was prepared by a serum pharmacology method. Human SGC-7901 cells were incubated with SPPW serum at three different concentrations and with the anticancer drug 5-fluorouracil (5-FU), respectively. Cell proliferation was assessed by MTT assay, and cell apoptosis was detected by flow cytometry assay. Real-time quantitative polymerase chain reaction (RT-PCR) and Western blot assay were employed to confirm the expressions of Bcl-2, Bax and p53 in SGC-7901 cells at mRNA and protein levels, respectively. Results SPPW serum suppressed the proliferation of SGC-7901 cells in a time- and dose-dependent manner. The colony forming rate of negative control was 48.2%, while those in the three SPPW serum groups and the 5-FU group decreased significantly (P Conclusions SPPW formula inhibits the proliferation of SGC-7901 cells in vitro and induces the cell apoptosis. It plays an anticancer role by regulating the expressions of Bax, p53 and Bcl-2 in SGC-7901 cells. Author Affiliation: (1) Department of Gastroenterology, the Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710004, China (2) Department of Emergency, the Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an, 710004, China Article History: Registration Date: 21/09/2012 Received Date: 08/06/2011 Online Date: 21/09/2012 Article note: Supported by "13115" Program of Shaanxi Province (No. 2007ZDKG-73)

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Gale Document Number: GALE|A322630754