Quantification of Aromatic Amino Acids in Cordyceps fungi by Micellar Electrokinetic Capillary Chromatography

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Date: June 2019
Publisher: Springer
Document Type: Report
Length: 273 words

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Byline: Xin Wei (1,2), Yaxia Su (1,2), Hankun Hu (1), Xiangtang Li (2), Rui Xu (2), Yiming Liu (2) Keywords: aromatic amino acids; micellar electrokinetic capillary chromatography; edible Cordyceps fungus; cultivated Cordyceps militaris; R 91 Abstract: A facile micellar electrokinetic chromatography (MEKC) method was developed for quantification of aromatic amino acids in Cordyceps fungi. The proposed MEKC method had linear calibration curves and detection limits at the [micro]mol/L level. Contents of aromatic amino acids in Cordyceps were found in the range from 0.004 9 % to 0.028 0% for tryptophan (Trp), 0.036 6% to 0.078 1% for tyrosine (Tyr), and 0.029 1% to 0.136 2% for phenylalanine (Phe). Levels of amino acids in cultivated Cordyceps militaris were found higher than those in natural Cordyceps sinensis. Interestingly, the ratio of Tyr to its precursor, Phe, in C. sinensis (1.20 [+ or -] 0.091, n=3) was significantly higher than that in cultivated C. militaris (0.54 [+ or -] 0.170, n=3). This is likely because the enzyme-catalyzed in vivo biotransformation of Phe to Tyr is much more effective in natural C. sinensis, a biological hybrid of larva and parasitic fungus, than in cultivated C. militaris. Author Affiliation: (1) grid.413247.7, Department of Pharmacy, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, China (2) 0000 0001 0671 8898, grid.257990.0, Department of Chemistry and Biochemistry, Jackson State University, Jackson, Mississippi, 39217, USA Article History: Registration Date: 13/05/2019 Received Date: 07/12/2018 Online Date: 14/05/2019 Article note: Foundation item: Supported by Zhongnan Hospital of Wuhan University Science, Technology, and Innovation Seed Fund (cxpy2017023), and the TCM Research Project of Hubei Provincial Health Commission (ZY2019M033) These authors contribute equally to this study

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Gale Document Number: GALE|A585460350