Apaf-1 and caspase-9 do not act as tumor suppressors in myc-induced lymphomagenesis or mouse embryo fibroblast transformation

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From: The Journal of Cell Biology(Vol. 164, Issue 1)
Publisher: Rockefeller University Press
Document Type: Author abstract
Length: 117 words

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Abstract :

Based on experiments with cultured fibroblasts, the apoptosis regulators caspase-9 and Apaf-1 are hypothesized to function as tumor suppressors. To investigate their in vivo role in lymphomagenesis, an IgH enhancer-driven c-myc transgene was crossed onto Apaf-[1.sup.-/-] and caspase-[9.sup.-/-] mice. Due to perinatal lethality, E[micro]-myc transgenic Apaf-[1.sup.-/-] or caspase-[9.sup.-/-] fetal liver cells were used to reconstitute lethally irradiated recipient mice. Surprisingly, no differences were seen in rate, incidence, or severity of lymphoma with loss of Apaf-1 or caspase-9, and Apaf-1 was not a critical determinant of anticancer drug sensitivity of c-myc-induced lymphomas. Moreover, loss of Apaf-1 did not promote oncogene-induced transformation of mouse embryo fibroblasts. Thus, Apaf-1 and caspase-9 do not suppress c-myc-induced lymphomagenesis and embryo fibroblast transformation.

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Gale Document Number: GALE|A114921853