Fluorescence dilution technique for measurement of albumin reflection coefficient in isolated glomeruli

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Publisher: American Physiological Society
Document Type: Author abstract; Report
Length: 250 words

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Abstract :

This study describes a highthroughput fluorescence dilution technique to measure the albumin reflection coefficient (aAib) of isolated glomeruli. Rats were injected with FITC-dextran 250 (75 mg/kg), and the glomeruli were isolated in a 6% BSA solution. Changes in the fluorescence of the glomerulus due to water influx in response to an imposed oncotic gradient was used to determine kidney; glomerulus; proteinuria; renal hemodynamics; renal disease doi: 10.1152/ajprenal.00311.2015.

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Gale Document Number: GALE|A442285870