Discovery of deoxyribonuclease II-like proteins in bacteria.

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Date: Sept. 2022
Publisher: Elsevier B.V.
Document Type: Report; Brief article
Length: 390 words

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Abstract :

Keywords DNase II; Phylogenetic; DNase activity; Defense islands; Anti-viral Highlights * Wide-spread presence of DNase II-like proteins in bacteria. * Sequence, phylogenetic and structural analyses show that bacterial candidate sequences possess eukaryotic DNase II characteristics. * DNase zymogram activity assay detects acidic DNase activities in Chromobacterium violaceum cell lysates which verifies the in silico analyses. * A possible involvement of DNase II as a part of an anti-phage defense system in bacteria. Abstract Deoxyribonuclease II (DNase II) is one of the earliest enzymes discovered in the history of biochemistry. Its role in apoptosis and development has been documented with great detail in eukaryotes. Prior in silico analyses showed its complete absence in bacterial genomes, with the exception of single bacterial genus: Burkholderia. It is therefore considered to be a eukaryotic enzyme. Here we show that the presence of DNase II is not limited to Burkholderia, as we find over one hundred DNase II-like sequences spanning 90 bacteria species belonging to 54 different genera and seven phyla. The majority of the significant hits (85%) come from Bacteroidetes and Proteobacteria phyla. Sequence analyses reveal that bacterial DNase II-like proteins possess a signature catalytic motif of eukaryotic DNase II. In phylogenetic analyses, we find that bacterial DNase II-like proteins are divided into two distinct clades. Our structural analyses reveal high levels of similarity between experimentally determined crystal structures of recombinant Burkholderia thailandensis DNase II and candidate bacterial DNase II-like proteins. We also biochemically show that Chromobacterium violaceum cell lysate possesses acidic DNase II-like activities. Collectively, our results indicate that DNase II has deeper evolutionary roots than previously thought. We argue that either some prokaryotic lineages have undergone losses of DNase II genes, resulting in rare conservation, or some lineages have acquired DNase II genes from eukaryotes through lateral gene transfer. We also discuss the possible involvement of DNase II as a part of an anti-phage defense system in bacteria. Author Affiliation: (a) Department of Biology, Institute of Graduate Programs, Eskisehir Technical University, Iki Eylul Campus, 26555 Eskisehir, Turkey (b) Department of Biology, Faculty of Sciences, Eskisehir Technical University, Yunus Emre Campus, 26470 Eskisehir, Turkey * Corresponding author at: Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA. Article History: Received 24 February 2022; Revised 8 May 2022; Accepted 16 May 2022 Byline: Erhan Aslan [ea3024@cumc.columbia.edu] (a,*), Muhittin Arslanyolu (b)

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Gale Document Number: GALE|A708782316