Prostaglandin E.sub.2 is crucial in the response of podocytes to fluid flow shear stress

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Publisher: Springer
Document Type: Report
Length: 350 words

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Byline: Tarak Srivastava (1), Ellen T. McCarthy (2), Ram Sharma (3), Patricia A. Cudmore (1), Mukut Sharma (4), Mark L. Johnson (5), Lynda F. Bonewald (5) Keywords: Prostaglandin E.sub.2; Cyclooxygenase; Actin; Mechanical strain; Shear stress Abstract: Podocytes play a key role in maintaining and modulating the filtration barrier of the glomerulus. Because of their location, podocytes are exposed to mechanical strain in the form of fluid flow shear stress (FFSS). Several human diseases are characterized by glomerular hyperfiltration, such as diabetes mellitus and hypertension. The response of podocytes to FFSS at physiological or pathological levels is not known. We exposed cultured podocytes to FFSS, and studied changes in actin cytoskeleton, prostaglandin E.sub.2 (PGE.sub.2) production and expression of cyclooxygenase-1 and--2 (COX-1, COX-2). FFSS caused a reduction in transversal F-actin stress filaments and the appearance of cortical actin network in the early recovery period. Cells exhibited a pattern similar to control state by 24 h following FFSS without significant loss of podocytes or apoptosis. FFSS caused increased levels of PGE.sub.2 as early as 30 min after onset of shear stress, levels that increased over time. PGE.sub.2 production by podocytes at post-2 h and post-24 h was also significantly increased compared to control cells (p Author Affiliation: (1) Section of Nephrology, Children's Mercy Hospital and University of Missouri at Kansas City, Kansas City, MO, USA (2) Kidney Institute, University of Kansas Medical Center, Kansas City, KS, USA (3) Renal Research Laboratory, Research and Development, Kansas City VA Medical Center, Room F1-130, Building 15, 4801 Linwood Boulevard, Kansas City, MO, 64128, USA (4) Kidney Disease Center, Medical College of Wisconsin, Milwaukee, WI, USA (5) Department of Oral Biology, University of Missouri at Kansas City--School of Dentistry, Kansas, MO, USA Article History: Registration Date: 05/03/2010 Received Date: 17/09/2009 Accepted Date: 05/03/2010 Online Date: 08/04/2010

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Gale Document Number: GALE|A228130328