Trilobatin Protects Cardiomyocytes from Hypoxia/Reperfusion Injury by Regulating the Nuclear Factor Erythroid 2-Related Factor 2/Heme Oxygenase-1 Pathway.

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Authors: Wenyu Chen and Hui He
Date: May 2021
Publisher: New Century Health Publishers, LLC
Document Type: Article
Length: 3,469 words
Lexile Measure: 1300L

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Abstract :

Trilobatin is a natural plant-derived glycosylated flavonoid that has been shown to exhibit multiple beneficial pharmacologic activities including protection of heart against H/R-induced cardiomyocyte injury. However, the molecular mechanisms underlying protection from H/R-induced cardiomyocyte injury remain unknown. Using H9C2 cells as a model, we examined the effect of trilobatin on H/R-induced cellular injury, apoptosis, and generation of reactive oxygen species. The results showed that trilobatin protected H9C2 cells not only from cell death and apoptosis, but also counteracted H/R-induced changes in malondialdehyde, superoxide dismutase, glutathione, and glutathione peroxidase. The evaluation of the mechanism underlying the effect of trilobatin on protection from H/R-induced cellular injury suggested changes in the regulation of nuclear factor erythroid 2-related factor 2/heme oxygenase-1 pathway. Keywords: Apoptosis, Cardiomyocyte, Hypoxia/Reperfusion, Nrf2/Ho-1, Trilobatin Abbreviations Used: Analysis of variance, ANOVA; Bicinchoninic acid, BCA; Cell Counting Kit-8, CCK-8; Dulbecco's modified Eagle's medium, DMEM; Enzyme linked immunosorbent assay, ELISA; Glyceraldehyde 3-phosphate dehydrogenase, GAPDH; Glutathione peroxidase, GSH-Px; Reduced glutathione, GSH; Hypoxia/reperfusion, H/R; Heme oxygenase-1, HO-1; Lactic dehydrogenase, LDH; Malondialdehyde, MDA; Nuclear factor erythroid 2-related factor 2, Nrf-2; Phosphate-buffered saline, PBS; Polymerase chain reaction, PCR; Quantitative polymerase chain reaction, qPCR; Radioimmunoprecipitation assay buffer, RIPA buffer; Reactive oxygen species, ROS; Real time polymerase chain reaction, RT-PCR; Standard deviation, SD; Sodium dodecyl sulfate polyacrylamide gel-electrophoresis, SDS-PAGE; Superoxide dismutase, SOD; Annexin V/Propidium Iodide double staining, V/PI double staining Corresponding Author: Dr. Hui He, Department of ECG Diagnosis, ZheJiang Hospital, No. 12 Lingyin road, Hangzhou City, Zhejiang Province, China; Email: huih777@163.com

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Gale Document Number: GALE|A659273144