Hyperin Ameliorates Proliferation, Migration, and Extracellular Matrix Formation in Airway Smooth Muscle Cells by Inhibiting Transforming Growth Factor-[beta]1-Induced Nuclear Factor-[kappa]B Activation.

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Publisher: New Century Health Publishers, LLC
Document Type: Article
Length: 3,279 words
Lexile Measure: 1480L

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Abstract :

We have explored the role of hyperin remodeling of airway smooth muscle cells during asthma. To this end, airway smooth muscle cells were isolated from tracheae and Pronchi of mice, treated with transforming growth factor-pi and increasing concentrations of hyperin followed by analysis of cell viability, proliferation, and migration. The levels of extracellular matrix formation were evaluated by analysis of fibronectin and type I collagen. Western blot analysis was used to assess the function of the downstream pathway of nuclear factor-kappa B. Transforming growth factor-pi treatment led to a dose-dependent increase in type I collagen and fibronectin that was reversed by hyperin. Transforming growth factor-pi promoted activation of nuclear factor-kappa B pathway with reduced kBa and enhanced phospho (p)-p65 and p-I[kappa]B[alpha]. However, hyperin treatment upregulated kBa and downregulated p-p65/p-kBa to inactivate NF-[kappa]B pathway. In conclusion, hyperin ameliorates proliferation, migration, and extracellular matrix formation in airway smooth muscle cells by inhibiting transforming growth factor-[beta]1-induced nuclear factor-kappa B activation suggesting potential prevention of airway remodeling during asthma. Keywords: Airway smooth muscle cells, Asthma, Hyperin, NF-[kappa]B, TGF-p1 Abbreviations Used: Protein kinase B, AKT; Bromodeoxyuridine, BrdU; Dimethyl sulfoxide, DMSO; Enhanced chemiluminescence, ECL; Fetal bovine serum, FBS; Inhibitor of nuclear factor kappa-B, I[kappa]B[alpha]; Mitogen-activated protein kinase, MAPK; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT; Nuclear factor-kappa B, NF-[kappa]B; Nuclear factor E2-related factor-2, Nrf2; A REL-associated protein involved in NF-[kappa]B heterodimer formation, nuclear translocation and activation, p65; Real-time quantitative reverse transcription PCR, qRT-PCR; Transforming growth factor-[beta]1, TGF-[beta]1 Corresponding Author: Dr. Weiwei Zhu, Department of Pediatrics, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, No. 105 Jiefang Road, Jinan, Shandong Province, China; E-mail: wwzhu666@163.com

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Gale Document Number: GALE|A659273158