Lupeol Inhibits Proliferation and Promotes Apoptosis of Ovarian Cancer Cells by Inactivating Phosphoinositide-3-Kinase/Protein Kinase B/Mammalian Target of Rapamycin Pathway.

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Authors: Feng Chen and Bei Zhang
Date: May 2021
Publisher: New Century Health Publishers, LLC
Document Type: Article
Length: 3,008 words
Lexile Measure: 1330L

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Abstract :

Lupeol exhibits multiple pharmacological activities including, anticancerous, anti-inflammatory, and antioxidant. The aim of this study was to explore the anticancerous activity of lupeol on ovarian cancer cells and examine its mechanism of action. To this end, increasing concentrations of lupeol on cell viability, cell cycle, and apoptosis in Caov-3 cells were evaluated. Lupeol inhibited cell viability, induced G1 phase arrest in cell cycle, increased cell apoptosis, and inhibited the ratio of phospho-Akt/protein kinase B and phospho-mammalian target of rapamycin/mammalian target of rapamycin. In conclusion, these data suggest that lupeol may play a therapeutic role in ovarian cancer. Keywords: Lupeol, Mammalian target of rapamycin, Ovarian cancer, Phosphatidylinositol-3-kinase, Protein kinase B Abbreviations Used: Protein kinase B, AKT; Ovarian cancer cells, Caov-3; Cell counting kit-8, CCK-8; Fluorescein-isothiocyanate, FITC; Hepatocellular carcinoma, HCC; Mammalian target of rapamycin, mTOR; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT; Phospho-Akt, p-Akt; Phospho-mTOR, p-mTOR; Propidium iodide, PI; Phosphoinositide-3-kinase, PI3K; Sodium dodecyl sulfate polyacrylamide gel-electrophoresis, SDS-PAGE Corresponding Author: Dr. Bei Zhang, Department of Obstetrics and Gynecology, Xuzhou Central Hospital affiliated to Nanjing University of Traditional Chinese Medicine, No. 199 Jiefang Nan Lu, Quanshan District, Xuzhou City, Jiangsu Province, China; E-mail:

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Gale Document Number: GALE|A659273155